[Solid phase extraction of BSA].

Area of Science:

• Analytical Chemistry
• Materials Science

Background:

• Solid phase extraction (SPE) is a crucial technique for sample purification.
• Developing efficient adsorbents is key for improving SPE performance.
• Bovine serum albumin (BSA) is a common protein analyte in biological and food samples.

Purpose of the Study:

• To investigate silica immobilized with polyoxyethyle-neisooctylphenol (SiO2-TX) as a novel adsorbent for SPE.
• To evaluate the efficiency of SiO2-TX for extracting bovine serum albumin (BSA).

Main Methods:

• Preparation of SiO2-TX adsorbent.
• Solid phase extraction experiments using SiO2-TX.
• Optimization of extraction conditions, including pH and surfactant type.

Main Results:

• SiO2-TX demonstrated high efficiency for BSA extraction, achieving up to 96% recovery.
• Efficient extraction was observed from aqueous solutions.
• BSA extraction was effective through the formation of ionic associates with both cationic and anionic surfactants at pH 8 and 1.5, respectively.

Conclusions:

• SiO2-TX is a promising adsorbent material for the solid phase extraction of BSA.
• The method is effective in aqueous solutions and adaptable to different surfactant types and pH conditions.