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Extracellular Vesicle Tissue Factor Activity Assay
Published on: December 29, 2023
Evaluation of tissue factor expression in canine tumor cells
Tracy Stokol1, Janelle L Daddona, Lamya S Mubayed
1Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA. ts23@cornell.edu
American Journal of Veterinary Research
|August 2, 2011
Summary
Canine tumor cells express tissue factor (TF) and shed TF-containing microparticles. High TF expression in canine tumors may influence their growth and spread, similar to human cancers.
Area of Science:
- Oncology
- Hematology
- Molecular Biology
Background:
- Tissue factor (TF) is a key initiator of the coagulation cascade.
- Tumor-associated TF is implicated in cancer progression and metastasis in humans.
- The role of TF in canine cancers is not well understood.
Purpose of the Study:
- To investigate the expression and functionality of tissue factor in canine tumor cell lines.
- To determine if canine tumor cells shed tissue factor-containing microparticles.
Main Methods:
- Utilized various canine tumor cell lines (mammary, pancreas, lung, prostate, bone, soft tissue) and control cells.
- Assessed tissue factor mRNA and protein expression using RT-PCR, flow cytometry, and immunofluorescence.
- Measured procoagulant activity of tissue factor on cell surfaces, lysates, and microparticles.
Main Results:
- All canine tumor cell lines expressed tissue factor mRNA and intracellular antigen.
- Canine mammary and lung tumor cells showed the highest tissue factor expression and activity.
- These tumor cells also shed tissue factor-bearing microparticles.
Conclusions:
- Canine tumor cell lines exhibit constitutive high expression of tissue factor.
- Epithelial-derived canine tumors, particularly mammary and lung, show significant TF activity.
- High TF expression in canine tumors may impact their in vivo behavior, including growth and metastasis.
