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Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
Sanger Sequencing01:57

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Genomics02:02

Genomics

Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...

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Updated: May 30, 2026

Sequencing of mRNA from Whole Blood using Nanopore Sequencing
11:26

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

High throughput DNA sequencing: The new sequencing revolution.

Michel Delseny1, Bin Han, Yue Ie Hsing

  • 1Laboratoire Génome et Développement des Plantes, UMR5096 CNRS-IRD-UP, University of Perpignan, 58 av. Paul Alduy, 66860 Perpignan, France. delseny@univ-perp.fr

Plant Science : an International Journal of Experimental Plant Biology
|August 2, 2011
PubMed
Summary

Technological advancements in DNA sequencing are revolutionizing plant science, enabling new research and breeding strategies. This review covers sequencing methods, plant genome achievements, and their impact on biodiversity and agriculture.

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G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

Area of Science:

  • Plant Science
  • Genomics
  • Bio-medical Research

Background:

  • Rapid technological advancements in DNA sequencing are transforming biological research.
  • Plant science has significantly benefited from these breakthroughs, leading to increased availability of plant genomes.

Purpose of the Study:

  • To review the principles of new DNA sequencing technologies.
  • To summarize the current state and recent achievements in plant genome sequencing.
  • To discuss the impact of genome sequencing on plant biodiversity, gene expression, and epigenetics.

Main Methods:

  • Description of novel sequencing methodologies.
  • Review of published plant genome sequencing projects and findings.
  • Analysis of discoveries in plant genome structure, evolution, and regulation.

Main Results:

  • Numerous plant genomes have been sequenced, opening new avenues for research.
  • Significant progress has been made in understanding plant genome structure and evolution.
  • Genome sequencing has influenced plant breeding and biodiversity conservation efforts.

Conclusions:

  • The field of plant genomics is entering a new era driven by advanced sequencing technologies.
  • Genome sequencing provides crucial insights for improving plant breeding and protecting biodiversity.
  • Future trends, challenges, and perspectives in plant genome sequencing are discussed.