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Image-guided, Laser-based Fabrication of Vascular-derived Microfluidic Networks
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SAM-based cell transfer to photopatterned hydrogels for microengineering vascular-like structures.

Nasser Sadr1, Mojun Zhu, Tatsuya Osaki

  • 1Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA 02139, USA.

Biomaterials
|August 2, 2011
PubMed
Summary

This study introduces a novel method for engineering vascular structures using self-assembled monolayer (SAM)-based cell transfer and hydrogel photopatterning. This technique enables efficient transfer of endothelial cells, paving the way for advanced tissue engineering.

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Last Updated: May 30, 2026

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Cell Biology

Background:

  • Reproducing native 3D microvascular architecture is crucial for functional tissue engineering.
  • Current methods struggle to create perfusable vessels with native 3D structural organization.

Purpose of the Study:

  • To develop a new method for microengineering vascular structures by combining self-assembled monolayer (SAM)-based cell transfer and gelatin methacrylate hydrogel photopatterning.
  • To investigate photoinduced and electrochemically triggered SAM desorption mechanisms for cell transfer.
  • To engineer vascular-like structures and mimic smooth muscle layers for tissue engineering applications.

Main Methods:

  • Utilized self-assembled monolayer (SAM)-based cell transfer and gelatin methacrylate hydrogel photopatterning.
  • Investigated photoinduced and electrochemically triggered SAM desorption mechanisms for human umbilical vein cell (HUVEC) transfer.
  • Engineered 3D vascular-like structures and photopatterned cell-laden hydrogel layers.

Main Results:

  • Achieved efficient (>97%) HUVEC monolayer transfer using photoinduced SAM desorption during hydrogel photocrosslinking.
  • Demonstrated preserved cell morphology and high transfer efficiency with electrochemically triggered desorption over extended culture periods.
  • Successfully engineered and maintained 3D vascular-like structures in perfusion culture for 15 days, including mimicking smooth muscle layers.

Conclusions:

  • The combination of SAM-based cell transfer and hydrogel photocrosslinking offers a promising approach for microengineering vascular structures.
  • This method facilitates the creation of perfusable vascular networks with improved structural organization.
  • The developed techniques hold potential for advancing regenerative medicine and tissue engineering by enabling the construction of more complex vascularized tissue constructs.