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Updated: May 30, 2026

Characterization of Thymus-dependent and Thymus-independent Immunoglobulin Isotype Responses in Mice Using Enzyme-linked Immunosorbent Assay
Published on: September 7, 2018
Important parameters for evaluation of antibody avidity by immunosorbent assay
Jordan D Dimitrov1, Sebastien Lacroix-Desmazes, Srinivas V Kaveri
1INSERM U872, Paris F-75006, France. jordan.dimitrov@crc.jussieu.fr
Abstract:
The evaluation of antibody avidity by elution with chaotropic agents is a frequently used approach in research and diagnostics. It provides important information on the functional relevance of antibodies. However, in the literature, there is a large heterogeneity in the experimental settings for the determination of this important parameter. Here, we demonstrate that antibody concentration and the choice of chaotropic agent are critical for the reliable estimation of antibody avidity.
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