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In Vitro Culture of Epithelial Cells from Different Anatomical Regions of the Human Amniotic Membrane

In Vitro Culture of Epithelial Cells from Different Anatomical Regions of the Human Amniotic Membrane

Published on: November 28, 2019

An epidermal stem cells niche microenvironment created by engineered human amniotic membrane.

Shi-zhao Ji¹, Shi-chu Xiao, Peng-fei Luo

¹Burns Institute of People's Liberation Army, Changhai Hospital, The Second Military Medical University, Shanghai 200433, People's Republic of China.

|August 2, 2011

Summary

Researchers developed micronized amniotic membrane (mAM) to rapidly amplify epidermal stem cells (ESCs) for skin tissue engineering. This novel scaffold promotes ESC expansion and supports skin defect repair in vivo.

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Isolation, Cryopreservation and Culture of Human Amnion Epithelial Cells for Clinical Applications

Isolation, Cryopreservation and Culture of Human Amnion Epithelial Cells for Clinical Applications

Published on: December 21, 2014

Area of Science:

Biomaterials Science
Tissue Engineering
Stem Cell Biology

Background:

Rapid amplification of epidermal stem cells (ESCs) is crucial for skin tissue engineering.
Current methods face challenges in efficiency and maintaining stem cell characteristics.

Purpose of the Study:

To develop a novel microcarrier for rapid ESC amplification.
To evaluate the efficacy of micronized amniotic membrane (mAM) in supporting ESC expansion and skin regeneration.

Main Methods:

Preparation of 3D micronized amniotic membrane (mAM) using freeze-thawing and homogenization.
Culture of ESCs on mAM within a rotary cell culture system (RCCS).
Assessment of ESC viability, differentiation, and stem cell marker retention.
In vivo transplantation of ESC-loaded mAM into full-thickness skin defects in nude mice.

Main Results:

mAM retained basement membrane structure and bioactive substances (e.g., growth factors).
mAM combined with RCCS significantly enhanced ESC amplification compared to 2D culture.
Cultured ESCs maintained stem cell characteristics and effectively regenerated epidermis and dermis in vivo.

Conclusions:

mAM serves as an effective natural microcarrier for ex vivo ESC culture and amplification.
mAM provides a suitable microenvironment mimicking the stem cell niche.
ESC-mAM constructs show potential as dermal scaffolds for skin substitutes in full-thickness defect repair.