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The term ribozyme is used for RNA that can act as an enzyme. Ribozymes are mainly found in selected viruses, bacteria, plant organelles, and lower eukaryotes. Ribozymes were first discovered in 1982 when Tom Cech’s laboratory observed Group I introns acting as enzymes. This was shortly followed by the discovery of another ribozyme, Ribonulcease P, by Sid Altman’s laboratory. Both Cech and Altman received the Nobel Prize in chemistry in 1989 for their work on ribozymes.
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MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria
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Published on: February 23, 2021

Compatibility and incompatibility in S-RNase-based systems.

Bruce McClure1, Felipe Cruz-García, Carlos Romero

  • 1Department of Biochemistry, Interdisciplinary Plant Group, 117 Schweitzer Hall, University of Missouri, Columbia, MO 65211, USA. mcclureb@missouri.edu

Annals of Botany
|August 2, 2011
PubMed
Summary

Self-incompatibility (SI) in plants relies on S-RNases and F-box proteins. Understanding how pollen avoids S-RNase cytotoxicity is key to unlocking plant reproduction mechanisms.

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Determination of Self-(In)compatibility and Inter-(In)compatibility Relationships in Citrus Using Manual Pollination, Microscopy, and S-Genotype Analyses
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Determination of Self-(In)compatibility and Inter-(In)compatibility Relationships in Citrus Using Manual Pollination, Microscopy, and S-Genotype Analyses

Published on: June 30, 2023

Area of Science:

  • Plant reproductive biology
  • Molecular genetics
  • Biochemistry

Background:

  • S-RNase-based self-incompatibility (SI) is crucial in Solanaceae, Rosaceae, and Plantaginaceae.
  • The S-locus controls compatibility, involving S-RNases in pistil rejection and S-locus F-box proteins in pollen.

Purpose of the Study:

  • To review the genetics of SI, focusing on S-RNases and pollen F-box proteins.
  • To explore modifier genes and mutations affecting SI in different plant families.
  • To discuss proposed mechanisms for compatibility and incompatibility.

Main Methods:

  • Review of existing literature on SI genetics and molecular mechanisms.
  • Analysis of mutation data in Solanum and Prunus species.
  • Discussion of proposed models for S-RNase action and pollen tube interaction.

Main Results:

  • S-RNases act as cytotoxins, with pollen tubes needing to evade their activity for compatibility.
  • Pollen-side mutations can cause SI breakdown, suggesting incompatibility may be the default.
  • In Prunus, S-locus F-box protein gene mutations directly lead to compatibility.

Conclusions:

  • Two models explain compatibility: S-RNase degradation or compartmentalization within the pollen tube.
  • These models offer different perspectives on whether compatibility or incompatibility is the default state.
  • Further identification and characterization of SI factors will clarify their roles in S-RNase-based SI.