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Related Concept Videos

NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
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Directionality of Nuclear Transport01:42

Directionality of Nuclear Transport

Ras-related nuclear protein or Ran is a small G protein that cycles between its GTP and GDP bound states. Ran specific regulators, a Ran GTPase Activating Protein or RanGAP present in the cytosol and a Ran guanine nucleotide exchange factor or RanGEF present inside the nucleus regulate GTP/GDP exchange. A high concentration of GTP inside the cells, in addition to this asymmetric distribution of  Ran-specific regulators, leads to a higher RanGTP concentration inside the nucleus. This...
TGF - β Signaling Pathway01:16

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors are of three kinds RI, RII, and RIII. The RI...
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MAPK Signaling Cascades

Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
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NF-kB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
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Interactions Between Signaling Pathways

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NF-&#954;B-dependent Luciferase Activation and Quantification of Gene Expression in Salmonella Infected Tissue Culture Cells
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NF-κB-dependent Luciferase Activation and Quantification of Gene Expression in Salmonella Infected Tissue Culture Cells

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The Ran-binding protein RanBPM can depress the NF-κB pathway by interacting with TRAF6.

Lan Wang1, Chengbo Fu, Yingbin Cui

  • 1Department of Biochemistry and Molecular Biology, National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, 5 Dong Dan San Tiao St, Beijing, 100005, China.

Molecular and Cellular Biochemistry
|August 2, 2011
PubMed
Summary
This summary is machine-generated.

Ran-binding protein in microtubule-organizing center (RanBPM) directly binds to TRAF6, influencing its ubiquitination and the NF-κB pathway. This interaction suggests RanBPM

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Area of Science:

  • Molecular Biology
  • Cell Signaling
  • Protein Interactions

Background:

  • Ran-binding protein in microtubule-organizing center (RanBPM) interacts with neurotrophin receptors.
  • Neurotrophin receptors p75NTR and TrkA interact with TRAF6.
  • Direct interaction between RanBPM and TRAF6 was previously unestablished.

Purpose of the Study:

  • To determine if RanBPM directly interacts with TRAF6.
  • To investigate the functional consequences of RanBPM-TRAF6 interaction on signaling pathways.

Main Methods:

  • Yeast two-hybrid system
  • Glutathione-S-transferase pull-down assays
  • Co-immunoprecipitation
  • Laser scanning confocal microscopy
  • Fluorescence resonance energy transfer

Main Results:

  • RanBPM directly binds to the C-terminus of TRAF6 via its SPRY motif.
  • Complex formation between RanBPM and TRAF6 confirmed in vitro and in mammalian cells.
  • RanBPM influences TRAF6 ubiquitination and NF-κB signaling pathway activation.

Conclusions:

  • RanBPM directly interacts with TRAF6.
  • RanBPM modulates TRAF6-mediated ubiquitination and NF-κB signaling.
  • RanBPM plays a role in gene transcription through its interaction with TRAF6.