Related Experiment Video
Updated: May 30, 2026
08:00
Sample Preparation and Relative Quantitation using Reductive Methylation of Amines for Peptidomics Studies
Published on: November 4, 2021
Quantitative peptidomics of mice lacking peptide-processing enzymes
Jonathan Wardman1, Lloyd D Fricker
1Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, NY 10461, USA. jonathan.wardman@phd.linstein.yu.edu
Methods in Molecular Biology (Clifton, N.J.)
|August 2, 2011
Summary
Peptidomics, the analysis of peptides, offers precise measurement of peptide forms and modifications. This study introduces a quantitative method using isotopic labels for multivariate analysis of tissue extracts.
Area of Science:
- Biochemistry
- Analytical Chemistry
- Proteomics
Background:
- Peptidomics analyzes peptides in tissue extracts using mass spectrometry.
- It precisely identifies peptide forms, including modifications, and distinguishes variants.
- Unlike other methods, it detects unknown peptides and hundreds in one experiment.
Purpose of the Study:
- To present a method for quantitative peptidomics.
- To enable multivariate analysis of multiple sample groups.
- To facilitate studies on gene knockout effects on peptide levels.
Main Methods:
- Quantitative peptidomics using mass spectrometry.
- Isotopic labeling with trimethylammonium butyrate in five forms.
- Single liquid chromatography/mass spectrometry run for analysis.
Main Results:
- The method allows precise measurement of peptide forms and modifications.
- Hundreds of peptides can be detected in a single experiment.
- Multivariate analysis of up to five sample groups is feasible.
Conclusions:
- This quantitative peptidomics approach provides detailed peptide analysis.
- The isotopic labeling method enables robust comparative studies.
- It is suitable for investigating biological changes affecting peptide profiles.
