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Related Experiment Video

Updated: May 30, 2026

The Examination of Peroxidase-Positive Leukocytes in Semen
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The Examination of Peroxidase-Positive Leukocytes in Semen

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[Semen analysis using methods of molecular genetics--a case report].

Patrick J Laberke1, Nadine Wiprächtiger, Holger Wittig

  • 1Institut für Rechtsmedizin der Universität Basel.

Archiv Fur Kriminologie
|August 3, 2011
PubMed
Summary

Molecular genetic methods successfully excluded a suspect in a sexual offense case. Despite initial negative tests, DNA analysis identified spermatozoa, leading to the exclusion of the primary suspect.

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Area of Science:

  • Forensic Science
  • Molecular Genetics
  • Genomic Analysis

Background:

  • Sexual offense investigations often rely on biological evidence.
  • Initial forensic tests, such as prostate-specific antigen (PSA) and cytological sperm analysis, can sometimes be inconclusive.
  • Advanced molecular techniques are crucial for sensitive detection and profiling of biological traces.

Observation:

  • A case report details the investigation of a sexual offense where initial forensic tests were negative.
  • Trace evidence was collected for analysis.
  • The victim had recent consensual sexual activity with her partner prior to the alleged incident.

Findings:

  • Differential lysis and DNA quantification methods were employed to analyze the trace evidence.

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Last Updated: May 30, 2026

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  • A small number of spermatozoa were successfully detected despite initial negative results.
  • DNA profiling of the detected spermatozoa matched the victim's boyfriend, not the initial suspect.

    • Implications:

    • This case highlights the utility of advanced molecular genetic techniques in forensic investigations, particularly when initial screening tests are negative.
    • Sensitive DNA analysis can resolve complex cases by accurately identifying individuals present in trace biological evidence.
    • The findings underscore the importance of considering recent consensual sexual activity in the interpretation of forensic evidence.