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Updated: May 30, 2026

High-Pressure NMR Experiments for Detecting Protein Low-Lying Conformational States
Published on: June 29, 2021
Note: Solution NMR probe for the study of CO2 sequestration at elevated pressure and temperature
J Diefenbacher1, J Piwowarczyk, R F Marzke
1Department of Physics, Arizona State University, Tempe, Arizona 85287-1504, USA.
A new nuclear magnetic resonance probe design enables studies of supercritical carbon dioxide (CO(2)) solutions. This advancement is crucial for understanding geological sequestration processes under high pressure and temperature conditions.
Area of Science:
- Chemistry
- Geochemistry
- Materials Science
Background:
- Nuclear magnetic resonance (NMR) is a powerful tool for studying chemical reactions and molecular motion.
- Standard NMR techniques are limited to moderate pressures and temperatures, hindering studies of supercritical CO(2).
- Supercritical CO(2) is relevant to geological and mineral sequestration, requiring specialized experimental conditions.
Purpose of the Study:
- To present a working design for a specialized variable-temperature NMR probe.
- To enable NMR studies of CO(2) solutions under supercritical conditions.
- To investigate the behavior of CO(2) in geological sequestration relevant environments.
Main Methods:
- Development of a specialized variable-temperature NMR probe with a pressure chamber.
- Utilizing (13)C NMR spectroscopy to analyze CO(2) solutions.
- Conducting measurements at pressures up to 70 atm and temperatures from 50 to 125 °C.
Main Results:
- Demonstrated the use of the probe in (13)C NMR measurements.
- Quantified bicarbonate and dissolved CO(2) fractions over time after pressurization.
- Determined steady-state fractions of these species as a function of temperature at constant pressure.
Conclusions:
- The developed NMR probe is effective for studying supercritical CO(2) solutions.
- The findings provide insights into CO(2) speciation under conditions relevant to geological sequestration.
- This technology facilitates advanced research in carbon capture and storage.
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