Studies on erythrocytes in malaria infected blood sample with Raman optical tweezers
Raktim Dasgupta1, Ravi Shanker Verma, Sunita Ahlawat
1Raja Ramanna Centre for Advanced Technology, Laser Biomedical Applications and Instrumentation Division, Indore-452013, India. raktim@rrcat.gov.in
Abstract:
Raman spectroscopy was performed on optically trapped red blood cells (RBCs) from blood samples of healthy volunteers (h-RBCs) and from patients suffering from P. vivax infection (m-RBCs). A significant fraction of m-RBCs produced Raman spectra with altered characteristics relative to h-RBCs. The observed spectral changes suggest a reduced oxygen-affinity or right shifting of the oxygen-dissociation curve for the intracellular hemoglobin in a significant fraction of m-RBCs with respect to its normal functional state.
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