Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 30, 2026

Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer
08:12

Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer

Published on: March 14, 2019

Sequencing circulating miRNA in maternal plasma with modified library preparation.

Qinyu Ge1, Hailing Li, Qi Yang

  • 1Key Laboratory of Child Development and Learning Science, Ministry of Education, Southeast University, Nanjing, 210096, China. geqinyu@seu.edu.cn

Clinica Chimica Acta; International Journal of Clinical Chemistry
|August 3, 2011
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Bacteria Colonization is Associated with Inflammation-Antigen Presentation Imbalance in Myeloid Cells and Tumor Microenvironment Features in Melanoma.

Immunological investigations·2026
Same author

Interactive effects of biochar, nitrogen fertilizer, and irrigation on SOC and crop yield in a rice-wheat rotation system.

Frontiers in plant science·2026
Same author

Transcriptomic profiling of exRNA from spent human embryonic-cleavage culture medium reveals potential in embryo quality assessment：a retrospective study.

Reproduction (Cambridge, England)·2026
Same author

Effectiveness of bilingual health education on obesity and dietary behaviors in Mongolian adolescents: a six-month intervention study.

Frontiers in public health·2026
Same author

Dissecting cellular heterogeneity across two ocular melanoma subtypes by single-cell RNA sequencing.

Melanoma research·2026
Same author

Age-driven marbling in Haimen goat meat: multi-omics dissection of lipid/amino signatures and IMF regulatory networks.

Food chemistry: X·2026

Modified sequencing methods improve detection of microRNAs (miRNAs) in maternal plasma. These cell-free nucleic acids show altered expression in twin pregnancies, suggesting potential as non-invasive diagnostic biomarkers.

Area of Science:

  • Biochemistry
  • Genomics
  • Maternal-Fetal Medicine

Background:

  • Circulating microRNAs (miRNAs) in maternal plasma are cell-free nucleic acids with potential for non-invasive prenatal diagnosis.
  • Next-generation sequencing (NGS) offers a promising approach for detecting these miRNAs.

Purpose of the Study:

  • To develop a modified library preparation method for SOLiD sequencing of maternal plasma miRNAs.
  • To analyze miRNA expression profiles in single and twin pregnancies.
  • To validate altered miRNA expression in twin pregnancies.

Main Methods:

  • A modified library preparation protocol for SOLiD sequencing was developed.
  • Maternal plasma miRNA was analyzed from single and twin pregnancies.
  • Quantitative PCR (qPCR) was used for comparative validation.

More Related Videos

Retrospective MicroRNA Sequencing: Complementary DNA Library Preparation Protocol Using Formalin-fixed Paraffin-embedded RNA Specimens
12:24

Retrospective MicroRNA Sequencing: Complementary DNA Library Preparation Protocol Using Formalin-fixed Paraffin-embedded RNA Specimens

Published on: May 5, 2018

Related Experiment Videos

Last Updated: May 30, 2026

Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer
08:12

Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer

Published on: March 14, 2019

Retrospective MicroRNA Sequencing: Complementary DNA Library Preparation Protocol Using Formalin-fixed Paraffin-embedded RNA Specimens
12:24

Retrospective MicroRNA Sequencing: Complementary DNA Library Preparation Protocol Using Formalin-fixed Paraffin-embedded RNA Specimens

Published on: May 5, 2018

Main Results:

  • The modified library preparation method significantly improved sequencing data quality.
  • Distinct miRNA expression patterns were observed in twin pregnancies compared to controls.
  • Several specific miRNAs showed significantly altered expression levels in twin pregnancies.

Conclusions:

  • MicroRNAs (miRNAs) likely play a role in pregnancy, including the development of twin pregnancies.
  • Specific circulating miRNAs may serve as potential biomarkers for clinical diagnosis and therapeutic strategies in pregnancy.