Related Experiment Video
Updated: May 30, 2026
Real-time In Vivo Recording of Arabidopsis Calcium Signals During Insect Feeding Using a Fluorescent Biosensor
Published on: August 15, 2017
"Off-On" based fluorescent chemosensor for Cu2+ in aqueous media and living cells
Chunwei Yu1, Lingxin Chen, Jun Zhang
1Key Laboratory of Coastal Zone Environmental Processes, CAS, Shandong Provincial Key Laboratory of Coastal Zone Environmental Processes, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, China.
Abstract:
A novel Cu(2+)-specific "off-on" fluorescent chemosensor of naphthalimide modified rhodamine B (naphthalimide modified rhodamine B chemosensor, NRC) was designed and synthesized, based on the equilibrium between the spirolactam (non-fluorescence) and the ring-opened amide (fluorescence). The chemosensor NRC showed high Cu(2+)-selective fluorescence enhancement over commonly coexistent metal ions or anions in neutral aqueous media. The limit of detection (LOD) based on 3×δ(blank)/k was obtained as low as 0.18 μM of Cu(2+), as well as an excellent linearity of 0.05-4.5 μM (R=0.999), indicating the chemosensor of high sensitivity and wide quantitation range. And also the coordination mode with 1:1 stoichiometry was proposed between NRC and Cu(2+). In addition, the effects of pH, co-existing metal ions and anions, and the reversibility were investigated in detail. It was also demonstrated that the NRC could be used as an excellent "off-on" fluorescent chemosensor for the measurement of Cu(2+) in living cells with satisfying results, which further displayed its valuable applications in biological systems.
