Association between border cell responses and localized root infection by pathogenic Aphanomyces euteiches
Marc Antoine Cannesan1, Christophe Gangneux, Arnaud Lanoue
1Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale, EA4358, IFRMP 23, Plate-forme de Recherche en Imagerie Cellulaire de Haute Normandie, Université de Rouen, 76821 Mont Saint Aignan, France.
Annals of Botany
|August 3, 2011
Summary
Pea plants
Area of Science:
- Plant pathology
- Plant-microbe interactions
- Oomycete diseases
Background:
- Aphanomyces euteiches causes significant crop loss in pea (Pisum sativum), leading to root growth arrest and plant death.
- Current disease control is limited to crop rotation, with no resistant pea varieties available.
- Understanding early oomycete-plant interactions is crucial for developing effective disease management strategies.
Purpose of the Study:
- To investigate the early stages of Aphanomyces euteiches root infection in pea at tissue and cellular levels.
- To analyze dynamic changes in plant secondary metabolism during oomycete-plant interactions.
- To elucidate the role of root border cells in plant defense against Aphanomyces euteiches.
Main Methods:
- Flow cytometry and microscopy were employed to study root infection dynamics.
- High-performance liquid chromatography with diode-array detection analyzed secondary metabolism changes.
- Oospore inoculation quantified the effect on border cell production.
Main Results:
- Root infection initiated in the elongation zone, sparing the root cap and border cells.
- Aphanomyces euteiches inoculation significantly enhanced border cell production, altering their morphology.
- Border cells increased pisatin synthesis, a phytoalexin, in response to pathogen challenge.
Conclusions:
- Pea root border cells exhibit distinct responses to Aphanomyces euteiches, indicating a role in local root tip defense.
- Root border cells provide a valuable quantitative model for studying plant-microbe interactions.
- Findings offer insights into pea's defense mechanisms against oomycete pathogens.
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