Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 30, 2026

Liquid-cell Transmission Electron Microscopy for Tracking Self-assembly of Nanoparticles
08:39

Liquid-cell Transmission Electron Microscopy for Tracking Self-assembly of Nanoparticles

Published on: October 16, 2017

A bacterial process for selenium nanosphere assembly.

Charles M Debieux1, Elizabeth J Dridge, Claudia M Mueller

  • 1Biosciences, College of Life and Environmental Sciences, University of Exeter, Stocker Road, Exeter EX4 4QD, United Kingdom.

Proceedings of the National Academy of Sciences of the United States of America
|August 3, 2011
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Microbe Profile: <i>Paracoccus denitrificans</i> - a versatile model.

Microbiology (Reading, England)·2026
Same author

Metabolic and bariatric surgery in adolescents compared to young adults: an MBSAQIP database analysis.

Surgery for obesity and related diseases : official journal of the American Society for Bariatric Surgery·2025
Same author

The scientific legacy of Stuart Ferguson.

Advances in microbial physiology·2025
Same author

Life on oxidised nitrogen: the biochemistry and physiology of prokaryotic life supported by the nitrate-nitrite redox couple.

Advances in microbial physiology·2025
Same author

Polarization-maintaining dissipative-soliton mode-locked thulium fiber laser with a nonlinear-amplifying-loop-mirror cavity operating at 1860 nm.

Optics express·2025
Same author

Laboratory and In-Field Metagenomics for Environmental Monitoring.

Methods in molecular biology (Clifton, N.J.)·2025

Thauera selenatis bacteria form selenium nanospheres during respiration. A novel protein, Se factor A (SefA), stabilizes these nanospheres, offering potential for bionanomaterial applications.

Area of Science:

  • Microbiology
  • Biochemistry
  • Nanotechnology

Background:

  • Thauera selenatis reduces selenate, forming intracellular selenium deposits.
  • These deposits are secreted as selenium nanospheres (approx. 150 nm).
  • A ~95 kDa protein is associated with these nanospheres.

Purpose of the Study:

  • To identify and characterize the protein associated with selenium nanospheres.
  • To investigate the role of this protein in selenium nanosphere formation.
  • To explore potential applications in bionanomaterial fabrication.

Main Methods:

  • Protein purification from selenium nanospheres.
  • Peptide mass spectrometry for gene identification in Thauera selenatis.
  • Gene cloning, expression, and purification of recombinant Se factor A (SefA) in E. coli.

More Related Videos

Facet-to-facet Linking of Shape-anisotropic Colloidal Cadmium Chalcogenide Nanostructures
09:12

Facet-to-facet Linking of Shape-anisotropic Colloidal Cadmium Chalcogenide Nanostructures

Published on: August 10, 2017

Seeded Synthesis of CdSe/CdS Rod and Tetrapod Nanocrystals
12:56

Seeded Synthesis of CdSe/CdS Rod and Tetrapod Nanocrystals

Published on: December 11, 2013

Related Experiment Videos

Last Updated: May 30, 2026

Liquid-cell Transmission Electron Microscopy for Tracking Self-assembly of Nanoparticles
08:39

Liquid-cell Transmission Electron Microscopy for Tracking Self-assembly of Nanoparticles

Published on: October 16, 2017

Facet-to-facet Linking of Shape-anisotropic Colloidal Cadmium Chalcogenide Nanostructures
09:12

Facet-to-facet Linking of Shape-anisotropic Colloidal Cadmium Chalcogenide Nanostructures

Published on: August 10, 2017

Seeded Synthesis of CdSe/CdS Rod and Tetrapod Nanocrystals
12:56

Seeded Synthesis of CdSe/CdS Rod and Tetrapod Nanocrystals

Published on: December 11, 2013

  • In vivo and in vitro assays for nanosphere formation and stabilization.

    • Main Results:

    • A ~95 kDa protein, named Se factor A (SefA), was identified and its gene characterized.
    • SefA is upregulated and secreted in response to selenite.
    • Recombinant SefA facilitated the formation of larger (approx. 300 nm) selenium nanospheres in E. coli.
    • SefA stabilizes selenium nanosphere formation in vitro.

    Conclusions:

    • SefA plays a crucial role in the assembly of selenium nanospheres.
    • The findings suggest SefA's potential utility in the fabrication of selenium bionanomaterials.