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Injectable fibroblast growth factor-2 coacervate for persistent angiogenesis.

Hunghao Chu¹, Jin Gao, Chien-Wen Chen

¹Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA 15261, USA.

Proceedings of the National Academy of Sciences of the United States of America

|August 3, 2011

Summary

This study developed an injectable coacervate to deliver fibroblast growth factor-2 (FGF2), significantly enhancing therapeutic angiogenesis. The new delivery system promotes mature blood vessel formation more effectively than free FGF2.

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Optimized Fibrin Gel Bead Assay for the Study of Angiogenesis

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Published on: April 29, 2007

Area of Science:

Biomaterials Science
Vascular Biology
Regenerative Medicine

Background:

Therapeutic angiogenesis requires mature vasculature, stabilized by mural cells and growth factors.
Effective delivery of growth factors like fibroblast growth factor-2 (FGF2) is crucial for successful angiogenesis.

Purpose of the Study:

To design and evaluate an injectable polyvalent coacervate for controlled delivery of FGF2 to enhance therapeutic angiogenesis.
To assess the efficacy of FGF2-loaded coacervates in promoting endothelial cell differentiation, pericyte chemotaxis, and in vivo blood vessel formation.

Main Methods:

Fabrication of an injectable polyvalent coacervate using a polycation, heparin, and FGF2.
In vitro assessment of FGF2 loading efficiency, protection from degradation, and biological activity (endothelial cell differentiation, pericyte chemotaxis).
In vivo evaluation of the coacervate's ability to induce angiogenesis after a single injection.

Main Results:

Nearly 100% loading efficiency of FGF2 into the coacervate, with protection from proteolytic degradation.
Coacervate-released FGF2 showed enhanced endothelial cell differentiation and pericyte chemotaxis compared to free FGF2.
A single injection of FGF2-loaded coacervate induced robust in vivo angiogenesis, characterized by increased endothelial and mural cells, larger blood vessels, and improved circulation.
Mature vasculature persisted for at least 4 weeks, unlike the weak response to free FGF2 injection.

Conclusions:

The polyvalent coacervate serves as an effective controlled delivery platform for FGF2.
This novel delivery system significantly enhances therapeutic angiogenesis by promoting vessel maturation and stability.
The findings highlight the potential of coacervates for developing advanced regenerative medicine strategies.