A novel and visual test for oral malodour: first observations

Area of Science:

• Biochemistry
• Biomarker Detection
• Oral Health Diagnostics

Background:

• Biogenic amines are potential biomarkers for oral malodour.
• Previous detection methods for these amines were complex, limiting clinical application.
• A need exists for simple, reliable chair-side diagnostic tools for halitosis.

Purpose of the Study:

• To explore the utility of a novel enzymatic colorimetric reaction for detecting salivary biogenic amines.
• To evaluate this test as an adjunct diagnostic tool for oral malodour.

Main Methods:

• Characterized a newly discovered enzyme's colorimetric reaction with amines in vitro.
• Developed 5- and 10-point pink color scales for quantifying amine concentrations.
• Assessed salivary amine levels in 50 volunteers using the enzymatic test and correlated results with organoleptic and volatile sulfur compound measurements.

Main Results:

• The enzymatic reaction demonstrated linearity with amine concentration and stability for at least 4 hours.
• Color scores from the test showed strong correlation with organoleptic scores and volatile sulfur compounds.
• Significant differences in amine levels were observed between individuals with and without oral malodour.

Conclusions:

• The new enzymatic test, interpreted via a simple color scale, is a viable adjunct for oral malodour diagnosis.
• This colorimetric method offers a practical chair-side solution for identifying halitosis based on biogenic amine levels.