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Related Experiment Video

Updated: May 30, 2026

Mouse Oocyte Microinjection, Maturation and Ploidy Assessment
07:03

Mouse Oocyte Microinjection, Maturation and Ploidy Assessment

Published on: July 23, 2011

Mouse oocyte microinjection, maturation and ploidy assessment.

Paula Stein1, Karen Schindler

  • 1Department of Biology, University of Pennsylvania, USA.

Journal of Visualized Experiments : Jove
|August 3, 2011
PubMed
Summary

This study details methods for isolating mouse oocytes and assessing aneuploidy, crucial for understanding infertility and developmental disorders. These techniques offer a more efficient approach to studying female meiosis errors.

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Area of Science:

  • Reproductive Biology
  • Cell Biology
  • Genetics

Background:

  • Aneuploidy in gametes causes infertility, miscarriages, and developmental disorders like Down syndrome.
  • Errors in the first meiotic division (MI) are a common source of aneuploidy in female meiosis.
  • In vitro manipulation of oocytes is an economical alternative to generating transgenic mouse models.

Purpose of the Study:

  • To describe methods for isolating prophase I oocytes from mice for microinjection.
  • To present a novel method for assessing both hypo- and hyperploidies in intact oocytes.
  • To detail in vitro maturation conditions for oocytes to the MII stage.

Main Methods:

  • Isolation of prophase-arrested oocytes and microinjection of cRNA.
  • In vitro maturation of oocytes to metaphase II (MII).

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Last Updated: May 30, 2026

Mouse Oocyte Microinjection, Maturation and Ploidy Assessment
07:03

Mouse Oocyte Microinjection, Maturation and Ploidy Assessment

Published on: July 23, 2011

Using Mouse Oocytes to Assess Human Gene Function During Meiosis I
11:13

Using Mouse Oocytes to Assess Human Gene Function During Meiosis I

Published on: April 10, 2018

Evaluation of the Spindle Assembly Checkpoint Integrity in Mouse Oocytes
10:09

Evaluation of the Spindle Assembly Checkpoint Integrity in Mouse Oocytes

Published on: September 13, 2022

  • Assessment of ploidy in intact eggs using monastrol to create a monopolar spindle and anti-CREST serum for kinetochore counting.

    • Main Results:

    • Established protocols for oocyte isolation and microinjection.
    • Validated an in vitro maturation system for oocytes.
    • Developed a robust method for detecting both hypo- and hyperploidies in intact oocytes, overcoming limitations of traditional chromosome spreading.

    Conclusions:

    • The described methods provide efficient tools for studying female meiosis and aneuploidy.
    • The novel ploidy assessment method enhances accuracy and reduces operator error.
    • These techniques facilitate research into the causes of infertility and developmental disorders linked to gamete aneuploidy.