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Related Concept Videos

Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview01:02

Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview

Ultraviolet–visible (UV–visible or UV–Vis) spectroscopy is an analytical technique that investigates the interaction between matter and UV–Vis light within the electromagnetic spectrum. This method is widely used for its versatility, simplicity, and relatively quick data acquisition, making it valuable for both qualitative and quantitative analysis. When UV–Vis radiation passes through a material,  molecules absorb light depending on the energy required for electronic transitions. As a result...
UV–Vis Spectrometers01:14

UV–Vis Spectrometers

The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell. Samples for...

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UV-Vis Spectroscopic Characterization of Nanomaterials in Aqueous Media
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Published on: October 25, 2021

Measuring silver nanoparticle dissolution in complex biological and environmental matrices using UV-visible

Justin M Zook1, Stephen E Long, Danielle Cleveland

  • 1Material Measurement Laboratory, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA. jzook@nist.gov

Analytical and Bioanalytical Chemistry
|August 3, 2011
PubMed
Summary

Measuring nanoparticle toxicity requires distinguishing nanoparticle effects from ion effects. A new localized surface plasmon resonance (LSPR) method accurately quantifies silver nanoparticle dissolution in complex solutions, overcoming limitations of existing techniques.

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Area of Science:

  • Environmental Science
  • Materials Science
  • Toxicology

Background:

  • Distinguishing nanoparticle toxicity from ion toxicity is crucial for nanotoxicity assessment and regulation.
  • Silver nanoparticles (AgNPs) pose a challenge due to silver ion transformation into silver chloride nanoparticles (AgCl NPs) in biological and environmental media.
  • Existing methods like ICP-MS cannot differentiate between AgNPs and AgCl NPs, hindering accurate dissolution measurements.

Purpose of the Study:

  • To develop a simple and accurate method for quantifying the amount of silver remaining in nanoparticle form.
  • To measure the dissolution rates of AgNPs in various biological and environmental solutions.
  • To assess the influence of AgNP concentration, coatings, and media composition on dissolution rates.

Main Methods:

  • Utilized localized surface plasmon resonance (LSPR) UV-visible absorbance spectroscopy to measure silver in AgNP form.
  • Validated the LSPR method against ICP-MS for AgNPs in non-chloride media.
  • Applied the LSPR method to determine AgNP dissolution rates in cell culture media and MHRW with varying AgNP concentrations and polymer coatings.

Main Results:

  • The LSPR absorbance method accurately quantifies AgNPs in the presence of chloride ions, as AgCl NPs lack LSPR.
  • AgNP dissolution rates decrease at higher AgNP concentrations.
  • Polyethylene glycol thiol coatings (5 kDa) increased AgNP dissolution rates, which were significantly higher in cell culture media than in MHRW.

Conclusions:

  • LSPR UV-visible absorbance offers a simple, effective technique for measuring AgNP dissolution in complex media without prior separation.
  • Environmental and biological factors significantly influence AgNP dissolution rates, impacting their toxicological profiles.
  • This method provides a critical tool for accurate nanotoxicity studies and regulatory assessments of silver nanoparticles.