Search research articles

Search research articles

Related Experiment Video

Updated: May 30, 2026

Non-invasive Optical Imaging of the Lymphatic Vasculature of a Mouse

Non-invasive Optical Imaging of the Lymphatic Vasculature of a Mouse

Published on: March 8, 2013

Demonstrating the lymphatic system in rats with microinjection.

Hiroo Suami¹, David W Chang, Kumiko Matsumoto

¹Department of Plastic Surgery, The University of Texas M. D. Anderson Cancer Center, Houston, TX 77030, USA. hsuami@mdanderson.org

Anatomical Record (Hoboken, N.J. : 2007)

|August 3, 2011

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Enhancing Diagnostic Precision and Treatment Effectiveness Using Indocyanine Green Lymphography in Lymphedema.

Annals of rehabilitation medicine·2026

Same author

Effects of monoglucosyl hesperidin on human lymphatic circulatory function: A randomized placebo-controlled, double-blind, crossover trial.

Scientific reports·2026

Same author

A new pneumatic lymphatic drainage device enhancing lymphatic flow in lower limbs with lymphedema: a single-center, single-arm, prospective, open-label, non-randomized, exploratory study.

Nagoya journal of medical science·2026

Same author

Indocyanine green lymphography imaging of normal lymphatic drainage in the lower limbs.

The British journal of radiology·2026

Same author

A Proposed Minimum Standard Set of Outcome Measures for Lymphatic Surgery: Results of a Modified Delphi Process from the Society of Lymphatic Surgery Leadership.

Plastic and reconstructive surgery·2026

Same author

Lymphedema: Current Concepts in Management.

Clinics in plastic surgery·2025

Same journal

Tracheal chambers as a key innovation for high-frequency emission in bat echolocation.

Anatomical record (Hoboken, N.J. : 2007)·2026

Same journal

PNA-lectin histochemistry shows putative epidermal cells secreting antifreeze glycoproteins (AFGPs) in the Antarctic fish Trematomus bernacchii (Boulenger, 1902).

Anatomical record (Hoboken, N.J. : 2007)·2026

Same journal

Cytoarchitectural and immunohistochemical characterization of calbindin and parvalbumin from the primary motor cortex of the marmoset (Callithrix jacchus).

Anatomical record (Hoboken, N.J. : 2007)·2026

Same journal

A second theropod tooth from the Cauvery Basin reaffirms the presence of troodontid dinosaurs in the Cretaceous of India.

Anatomical record (Hoboken, N.J. : 2007)·2026

Same journal

Scaling and ecomorphology of lagomorph body shape and appendicular skeleton.

Anatomical record (Hoboken, N.J. : 2007)·2026

Same journal

New fossil unveils the dentary anatomy of the rare lepidosauromorph Cargninia enigmatica from the Upper Triassic of Southern Brazil.

Anatomical record (Hoboken, N.J. : 2007)·2026

See all related articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Researchers developed a new microinjection technique using blue dye and hydrogen peroxide to visualize the rat lymphatic system. This method effectively maps lymphatic channels and lymph nodes, aiding cancer metastasis research.

Area of Science:

Anatomy
Physiology
Medical Imaging

Background:

The lymphatic system is crucial for immune response and cancer metastasis.
Visualizing the lymphatic system presents significant anatomical challenges.
Previous methods for lymphatic visualization include dissection and indirect dye injection.

Purpose of the Study:

To introduce a novel microinjection technique for visualizing the rat lymphatic system.
To demonstrate the utility of this method for mapping lymphatic channels and lymph nodes.
To provide a tool for advancing cancer metastasis research.

Main Methods:

Utilized a mixture of acrylic blue dye and hydrogen peroxide for visualization.
Cannulated lymphatic vessels with micropipettes in rats.

Keywords:

Gerota's method cancer metastasis lymphatic system mercury injection

More Related Videos

Microscopic Observation of Lymphocyte Dynamics in Rat Peyer's Patches

Microscopic Observation of Lymphocyte Dynamics in Rat Peyer's Patches

Published on: June 25, 2020

The Mesenteric Lymph Duct Cannulated Rat Model: Application to the Assessment of Intestinal Lymphatic Drug Transport

The Mesenteric Lymph Duct Cannulated Rat Model: Application to the Assessment of Intestinal Lymphatic Drug Transport

Published on: March 6, 2015

Related Experiment Videos

Last Updated: May 30, 2026

Non-invasive Optical Imaging of the Lymphatic Vasculature of a Mouse

Non-invasive Optical Imaging of the Lymphatic Vasculature of a Mouse

Published on: March 8, 2013

Microscopic Observation of Lymphocyte Dynamics in Rat Peyer's Patches

Microscopic Observation of Lymphocyte Dynamics in Rat Peyer's Patches

Published on: June 25, 2020

The Mesenteric Lymph Duct Cannulated Rat Model: Application to the Assessment of Intestinal Lymphatic Drug Transport

The Mesenteric Lymph Duct Cannulated Rat Model: Application to the Assessment of Intestinal Lymphatic Drug Transport

Published on: March 6, 2015

Injected radio-opaque orange lead oxide selectively into lymphatic vessels.

Main Results:

Successfully visualized the lymphatic system from extremities to subclavicular veins.
Identified key structures including lymph nodes, cisterna chyli, and thoracic duct.
Enabled both photographic and radiographic recording of lymphatic anatomy.

Conclusions:

The described microinjection technique offers an effective means to visualize the rat lymphatic system.
This method allows detailed mapping of lymphatic channels and lymph nodes.
The technique holds potential for enhancing research into cancer metastasis pathways.