Related Experiment Video
Updated: May 30, 2026
A Straightforward Method for Glucosinolate Extraction and Analysis with High-pressure Liquid Chromatography (HPLC)
Published on: March 15, 2017
[Inhibition of glucoraphenin enzymolysis in Raphani Semen by processing]
Wenhai Lv1, Tao Ren, Yongwen Su
1Shandong University of Traditional Chinese Medicine, Jinan 250355, China. luwenhaitcm@163.com
Objective:
To explore material basis of the pharmacological differences between the roasting and pro-roasting Raphani Semen.
Method:
The two new sulfur-containing compounds (A209 and B221) were found changed after processing in the water decoction. The common precursor-C3 of A209 and B211 and the precursor of C3 were seperated and purified. Their transforming relationship was proved.
Result:
The result showed that glucosinolates could decompose into sulforaphane and transform into A209 and B221 further in the boiling process.
Conclusion:
This study provides some experimental evidences for revealing the mechanism of Raphani Semen processing.
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