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An assay for quantifying infectious HIV particles.

E Tjøtta1, O Hungnes, B Grinde

  • 1Department of Virology, National Institute of Public Health, Oslo, Norway.

Journal of Virological Methods
|February 1, 1990
PubMed
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A new method quantifies infectious Human Immunodeficiency Virus (HIV) particles. This technique immobilizes virus-infected cells in agar gel for accurate antigen detection via immunofluorescence, enabling reliable viral load assessment.

Area of Science:

  • Virology
  • Immunology
  • Cell Biology

Background:

  • Accurate quantification of infectious viral particles is crucial for understanding viral pathogenesis and evaluating antiviral therapies.
  • Existing methods for Human Immunodeficiency Virus (HIV) quantification may have limitations in assessing infectious viral load.

Purpose of the Study:

  • To develop and validate a novel method for quantifying infectious Human Immunodeficiency Virus (HIV) particles in biological preparations.
  • To establish a reliable assay for determining viral infectivity applicable to cell-associated viruses.

Main Methods:

  • Developed a method involving mixing virus with susceptible cells to facilitate viral binding.
  • Immobilized virus-infected cells within an agar gel matrix to prevent further virus transfer.

Related Experiment Videos

  • Incubated cells for 4 days to allow expression of viral antigens.
  • Quantified infected cells using indirect immunofluorescence assay to determine the percentage of cells expressing viral antigens.

    • Main Results:

    • Successfully developed a method to assess the number of infectious viral particles.
    • Demonstrated that the method allows for the detection and quantification of HIV-infected cells through viral antigen expression.
    • The percentage of infected cells directly correlates with the initial infectious viral load.

    Conclusions:

    • The developed agar-based immunofluorescence assay provides a robust method for quantifying infectious HIV particles.
    • This technique is presumed to be adaptable for assessing infectivity of other viruses that replicate in non-adherent cells.
    • This assay offers a valuable tool for virology research and diagnostics.