Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 30, 2026

Ecotoxicological Effects of Microplastics on Bird Embryo Development by Hatching without Eggshell
08:11

Ecotoxicological Effects of Microplastics on Bird Embryo Development by Hatching without Eggshell

Published on: August 14, 2021

Media ionic strength impacts embryonic responses to engineered nanoparticle exposure.

Lisa Truong1, Tatiana Zaikova, Erik K Richman

  • 1Department of Environmental and Molecular Toxicology, The Sinnhuber Aquatic Research Laboratory and the Environmental Health Sciences Center at Oregon State University, Corvallis, OR 97333, USA.

Nanotoxicology
|August 4, 2011
PubMed
Summary

Zebrafish embryos tolerate low-ion environments. Adjusting media ionic strength prevents gold nanoparticle (AuNP) agglomeration, reducing toxicity and improving assessment accuracy for nanoparticle research.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Zebrafish behavioral assessment as a first-tier whole-organism NAM for developmental neurotoxicity: A multi-laboratory evaluation.

Neurotoxicology·2026
Same author

Integrated Chemical and Toxicity Screening of Tap Drinking Water across Western Oregon Using Suspect and Nontarget Screening.

Environmental science & technology·2026
Same author

Corrigendum to "Chemical structure drives developmental toxicity of alkyl-substituted naphthalenes in zebrafish" [Environ. Int. 204 (2025) 109837].

Environment international·2026
Same author

Morphological, Behavioral, and Transcriptomic Profiling Reveals Developmental Toxicity of PCB Metabolites in Zebrafish.

Toxics·2026
Same author

FINS: An interactive platform for automated zebrafish image analysis and morphological screening.

SLAS technology·2026
Same author

Discovery of INCB191358: A Potent and Selective DGKα/ζ Dual Inhibitor.

Journal of medicinal chemistry·2026

Area of Science:

  • Environmental Science
  • Toxicology
  • Nanotechnology

Background:

  • Gold nanoparticles (AuNPs) are increasingly used in biomedical applications.
  • Understanding nanoparticle behavior in biological systems is crucial for safety assessment.
  • Zebrafish embryos are a valuable model for in vivo toxicity studies.

Purpose of the Study:

  • To investigate the impact of solution ion concentrations on gold nanoparticle (AuNP) agglomeration.
  • To assess the in vivo biological responses of AuNPs in zebrafish embryos under varying ionic conditions.
  • To determine optimal media conditions for reliable AuNP toxicity testing.

Main Methods:

  • Embryonic zebrafish were exposed to 1.2-nm 3-mercaptopropionic acid-functionalized AuNPs (1.2-nm 3-MPA-AuNPs).

More Related Videos

Assessment of the Effects of Endocrine Disrupting Compounds on the Development of Vertebrate Neural Network Function Using Multi-electrode Arrays
08:28

Assessment of the Effects of Endocrine Disrupting Compounds on the Development of Vertebrate Neural Network Function Using Multi-electrode Arrays

Published on: April 26, 2018

Using Chicken Embryo as a Powerful Tool in Assessment of Developmental Cardiotoxicities
11:00

Using Chicken Embryo as a Powerful Tool in Assessment of Developmental Cardiotoxicities

Published on: March 21, 2021

Related Experiment Videos

Last Updated: May 30, 2026

Ecotoxicological Effects of Microplastics on Bird Embryo Development by Hatching without Eggshell
08:11

Ecotoxicological Effects of Microplastics on Bird Embryo Development by Hatching without Eggshell

Published on: August 14, 2021

Assessment of the Effects of Endocrine Disrupting Compounds on the Development of Vertebrate Neural Network Function Using Multi-electrode Arrays
08:28

Assessment of the Effects of Endocrine Disrupting Compounds on the Development of Vertebrate Neural Network Function Using Multi-electrode Arrays

Published on: April 26, 2018

Using Chicken Embryo as a Powerful Tool in Assessment of Developmental Cardiotoxicities
11:00

Using Chicken Embryo as a Powerful Tool in Assessment of Developmental Cardiotoxicities

Published on: March 21, 2021

  • AuNP agglomeration and zebrafish morbidity/mortality were monitored in solutions with varying ionic concentrations.
  • Minimum ion concentration for zebrafish embryonic development was established.

    • Main Results:

    • Zebrafish embryos showed no adverse effects in nearly ion-free media.
    • 1.2-nm 3-MPA-AuNPs rapidly agglomerated in low ionic strength solutions, inducing toxicity.
    • Dispersing AuNPs in high ionic strength media significantly reduced agglomeration and biological response.
    • Media ionic strength critically influences AuNP agglomeration rates and toxicity.

    Conclusions:

    • Zebrafish embryos are remarkably insensitive to external ion concentrations.
    • Ionic strength of exposure media is a key factor in AuNP dispersion and subsequent biological effects.
    • Adjusting media ionic strength is essential for accurate assessment of nanoparticle toxicity in vivo.