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Related Concept Videos

Scanning Electron Microscopy01:07

Scanning Electron Microscopy

A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
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Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...

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Related Experiment Video

Updated: May 30, 2026

User-friendly, High-throughput, and Fully Automated Data Acquisition Software for Single-particle Cryo-electron Microscopy
07:56

User-friendly, High-throughput, and Fully Automated Data Acquisition Software for Single-particle Cryo-electron Microscopy

Published on: July 29, 2021

A novel method for acquiring large-scale automated scanning electron microscope data.

A R Shiveley1, P A Shade, A L Pilchak

  • 1Air Force Research Laboratory, Materials and Manufacturing Directorate, WPAFB, OH 45433, USA. adam.shiveley@wpafb.af.mil

Journal of Microscopy
|August 4, 2011
PubMed
Summary
This summary is machine-generated.

New software using LabVIEW and AutoIT enables faster electron backscatter diffraction data collection over larger sample areas. This advancement offers greater flexibility than current commercial options for materials analysis.

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Last Updated: May 30, 2026

User-friendly, High-throughput, and Fully Automated Data Acquisition Software for Single-particle Cryo-electron Microscopy
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Published on: July 29, 2021

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Array Tomography Workflow for the Targeted Acquisition of Volume Information using Scanning Electron Microscopy

Published on: July 15, 2021

Area of Science:

  • Materials Science
  • Analytical Chemistry
  • Physics

Background:

  • Electron backscatter diffraction (EBSD) is a crucial technique for microstructural analysis.
  • Recent advancements in hardware and software have accelerated EBSD data acquisition rates.
  • Previous limitations included the time required to analyze large sample areas.

Purpose of the Study:

  • To develop and present novel software for enhanced electron backscatter diffraction analysis.
  • To increase the flexibility and efficiency of EBSD data collection.
  • To enable the analysis of significantly larger sample areas.

Main Methods:

  • Development of custom software using LabVIEW™ and AutoIT(©).
  • Integration of automated stage movements with conventional beam control.
  • Implementation of a user-friendly interface for increased operational flexibility.

Main Results:

  • The developed software significantly increases the rate of EBSD data acquisition.
  • Larger sample areas can be analyzed more efficiently compared to previous methods.
  • The custom code provides greater flexibility than existing commercial EBSD software solutions.

Conclusions:

  • The LabVIEW™ and AutoIT(©) software offers a flexible and efficient solution for large-area EBSD analysis.
  • This advancement facilitates more comprehensive microstructural characterization.
  • The open availability of the source code promotes further development and adoption in materials research.