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Enrichment and Detection of Clostridium perfringens Toxinotypes in Retail Food Samples
Published on: October 18, 2019
Clostridium perfringens tpeL is expressed during sporulation
Daniel Paredes-Sabja1, Nahid Sarker, Mahfuzur R Sarker
1Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, OR 97331, USA.
Microbial Pathogenesis
|August 4, 2011
Summary
Clostridium perfringens toxin TpeL is expressed during sporulation, regulated by key factors Spo0A and SigE. This toxin localizes to the mother cell compartment, indicating its role in sporulation.
Area of Science:
- Microbiology
- Bacterial pathogenesis
- Cellular biology
Background:
- Clostridium perfringens produces large clostridial toxins, including TpeL.
- Previous research confirmed TpeL expression during bacterial vegetative growth.
- TpeL expression during sporulation remained uncharacterized.
Purpose of the Study:
- To investigate the expression of Clostridium perfringens TpeL during sporulation.
- To determine the regulatory mechanisms controlling tpeL expression during sporulation.
- To identify the subcellular localization of TpeL in sporulating cells.
Main Methods:
- Quantitative reverse transcription PCR (qRT-PCR) to assess tpeL mRNA levels during sporulation.
- Analysis of tpeL expression in spo0A and sigE mutant strains.
- Fluorescence microscopy to determine TpeL localization using a TpeL-green fluorescent protein (GFP) fusion.
Main Results:
- TpeL is expressed during the sporulation phase of Clostridium perfringens.
- tpeL expression is dependent on the master sporulation regulator Spo0A.
- tpeL expression is also dependent on the sporulation-specific sigma factor SigE.
- TpeL protein is localized to the mother cell compartment of the sporulating cell.
Conclusions:
- TpeL is a novel sporulation-regulated toxin in Clostridium perfringens.
- The expression of TpeL during sporulation is controlled by Spo0A and SigE.
- TpeL's localization suggests a specific role in the mother cell during sporulation.
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