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Defining Hsp33's Redox-regulated Chaperone Activity and Mapping Conformational Changes on Hsp33 Using Hydrogen-deuterium Exchange Mass Spectrometry
Published on: June 7, 2018
Oxidative stress-induced proteome alterations target different cellular pathways in human myoblasts
Martin A Baraibar1, Janek Hyzewicz, Adelina Rogowska-Wrzesinska
1Laboratoire de Biologie Cellulaire du Vieillissement, UR4, Université Pierre et Marie Curie-Paris 6, 75252 Paris Cedex 05, France.
Free Radical Biology & Medicine
|August 4, 2011
Summary
Oxidative stress damages adult human muscle stem cells. This study identified key proteins altered by oxidative stress, revealing links to muscle disorders, cell death, and cancer.
Area of Science:
- Proteomics
- Cellular Biology
- Oxidative Stress Research
Background:
- Increased oxidative stress is linked to impaired adult human muscle stem cell function.
- Specific proteins involved in or damaged by oxidative stress in these cells remain largely unidentified.
Purpose of the Study:
- To identify proteins involved in the oxidative stress response.
- To analyze protein expression profiles and identify proteins oxidized by hydrogen peroxide.
- To understand the molecular networks affected by oxidative stress in muscle stem cells.
Main Methods:
- Parallel proteomics approach to analyze protein expression.
- Hydrogen peroxide-induced oxidative stress model.
- Mass spectrometry for protein identification.
- Immunodetection of protein carbonyl groups.
Main Results:
- Fifteen proteins involved in oxidative stress response were identified.
- Peroxiredoxins 1 and 6, glyceraldehyde-3-phosphate dehydrogenase, and α-enolase showed posttranslational modifications.
- Carbonylated proteins are primarily cytosolic, involved in metabolism, cellular homeostasis, and protein turnover.
- Pathway analysis highlighted skeletal/muscular disorders, cell death, and cancer networks, linked to p53 and huntingtin.
Conclusions:
- Oxidative stress significantly alters protein expression and carbonylation in muscle stem cells.
- Identified proteins and pathways provide insights into muscle disorders, aging, and cancer.
- Comprehensive proteomic analysis is crucial for understanding cellular responses to stress.
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