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Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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Simultaneous PET and multispectral 3-dimensional fluorescence optical tomography imaging system.

Changqing Li1, Yongfeng Yang, Gregory S Mitchell

  • 1Department of Biomedical Engineering, University of California, Davis, Davis, California 95616, USA. cqli@ucdavis.edu

Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine
|August 4, 2011
PubMed
Summary
This summary is machine-generated.

This study presents the first simultaneous positron emission tomography (PET) and 3D fluorescence optical tomography (FOT) system for in vivo molecular imaging. The integrated system demonstrates feasibility for simultaneous imaging in phantom and live animal studies.

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Area of Science:

  • Biomedical imaging
  • Molecular imaging
  • Optical tomography

Background:

  • Integrated PET and 3D FOT imaging offers unique advantages for in vivo molecular imaging.
  • Existing small-animal PET scanners can be adapted for simultaneous imaging.

Purpose of the Study:

  • To design, build, and evaluate a simultaneous PET and 3D FOT system.
  • To assess the feasibility of combined in vivo molecular imaging.

Main Methods:

  • A novel conical mirror was integrated into a small-animal PET scanner for simultaneous imaging.
  • The diffusion equation modeled optical photon propagation, with iterative reconstruction of 3D fluorescence images.
  • Experiments were conducted using phantoms and live animals.

Main Results:

  • PET had negligible impact on 3D FOT performance.
  • The conical mirror slightly reduced PET sensitivity and noise-equivalent count rate, increasing scatter fraction.
  • Successful simultaneous PET and FOT imaging was achieved in phantom and in vivo experiments.

Conclusions:

  • The developed system enables simultaneous PET and 3D FOT imaging.
  • This represents the first reported in vivo simultaneous PET-FOT results, demonstrating feasibility.