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Updated: May 30, 2026
Human Adipose Tissue Micro-fragmentation for Cell Phenotyping and Secretome Characterization
Published on: October 20, 2019
Collection and storage of human blood and adipose for genomic analysis of clinical samples
Ann K Cashion1, Reba A Umberger, Shirlean B Goodwin
1College of Nursing, University of Tennessee Health Science Center, 920 Madison, Suite 507 N, Memphis, TN 38163, USA.
Abstract:
In this methods article, we describe collection and storage of clinically acquired blood and adipose samples for transcript analysis in an ongoing study exploring obesity in renal transplant recipients. Total ribonucleic acid (RNA) was isolated from whole blood using the LeukoLOCK™ Total RNA Isolation System (n = 4), and comparisons between fresh and frozen samples were made. Abdominal subcutaneous adipose samples (n = 4) were obtained during kidney transplantation, flash frozen, and stored at -80°C. Adipose RNA was extracted using either the STAT-60 method modified for lipids or Trizol plus RNeasy extraction. Affymetrix HG-U133 plus 2.0 arrays and Affymetrix Human Gene 1.0 ST arrays were used for both blood and adipose transcriptome analysis. Purity, quality, and quantity of RNA were high with comparable results using both array platforms.
