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Updated: May 30, 2026
13:08
Quantitative Localization of a Golgi Protein by Imaging Its Center of Fluorescence Mass
Published on: August 10, 2017
Structure of Golgi transport proteins
Daniel Kümmel1, Karin M Reinisch
1Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06520, USA.
Cold Spring Harbor Perspectives in Biology
|August 5, 2011
Summary
Golgi apparatus function relies on membrane transport, involving proteins like Arf GTPases and SNAREs. Structural studies reveal how these components interact to mediate vesicle trafficking within the cell.
Area of Science:
- Cell Biology
- Molecular and Structural Biology
Background:
- The Golgi apparatus is central to cellular function, requiring continuous vesicular transport for its operations.
- Membrane dynamics, including fission and fusion, are essential for vesicular transport within the Golgi.
- Key molecular players like Arf GTPases, coat proteins, Rabs, tethers, and SNAREs orchestrate these transport events.
Purpose of the Study:
- To elucidate the structural basis of Golgi membrane traffic.
- To investigate the interactions between Golgi coats, tethers, SNAREs, and GTPases.
- To provide insights into the mechanisms governing vesicular transport within the Golgi apparatus.
Main Methods:
- Detailed structural studies of Golgi-associated protein complexes.
- Analysis of interactions between coat proteins, tethers, SNAREs, and GTPases.
- Integration of structural data with functional insights into membrane traffic.
Main Results:
- Provided high-resolution structures of key Golgi coat and tethering factors.
- Revealed specific interaction interfaces between these factors and SNAREs/GTPases.
- Offered mechanistic explanations for how these interactions facilitate membrane fission and fusion.
Conclusions:
- Structural insights are crucial for understanding the molecular machinery of Golgi vesicular transport.
- The interplay between coats, tethers, SNAREs, and GTPases is finely tuned for efficient membrane traffic.
- This work advances our comprehension of fundamental cellular processes mediated by the Golgi apparatus.
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