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Updated: May 30, 2026
13:08
Quantitative Localization of a Golgi Protein by Imaging Its Center of Fluorescence Mass
Published on: August 10, 2017
Golgi and related vesicle proteomics: simplify to identify
Joan Gannon1, John J M Bergeron, Tommy Nilsson
1The Research Institute of the McGill University Health Centre and the Department of Medicine, Montreal, Quebec, Canada.
Cold Spring Harbor Perspectives in Biology
|August 5, 2011
Summary
Investigating Golgi transport mechanisms, this study explores multiple models, including vesicular and cisternal progression, using advanced proteomics. Proteomics offers insights into secretory pathway composition and protein transport, complementing other research methods.
Area of Science:
- Cell Biology
- Molecular Biology
- Biochemistry
Background:
- The fundamental mechanisms of biosynthetic material transport through the secretory pathway, specifically the Golgi apparatus, remain incompletely understood despite extensive research.
- The Golgi apparatus exhibits remarkable plasticity, maintaining structure and function even when perturbed, a characteristic revealed by advanced imaging techniques.
- Multiple models for protein transport within the Golgi exist, but a definitive consensus on the primary mechanism is lacking.
Purpose of the Study:
- To explore and evaluate various models of protein transport within the Golgi apparatus.
- To investigate the role of emerging technologies, particularly mass spectrometry-based proteomics, in elucidating Golgi transport mechanisms.
- To highlight the complementary nature of proteomics when integrated with other research methodologies.
Main Methods:
- Live cell imaging and correlative microscopy to observe Golgi dynamics.
- Single molecule and super-resolution microscopy for detailed structural analysis.
- Mass spectrometry-based proteomics to analyze the composition of Golgi compartments and subcompartments.
Main Results:
- Proteomics has provided insights into the relative composition of secretory pathway compartments.
- Mass spectrometry-based proteomics aids in understanding mechanistic aspects of protein transport within the Golgi.
- The study emphasizes that proteomics is most effective when used in conjunction with complementary experimental approaches.
Conclusions:
- Multiple mechanisms, including vesicular transport, cisternal progression/maturation, tubular connections, and inter-Golgi stack transport, are considered for Golgi transport.
- Mass spectrometry-based proteomics is a valuable, emerging technology for dissecting Golgi function and protein transport.
- Integrating proteomics with other advanced techniques is crucial for a comprehensive understanding of the secretory pathway.
Related Concept Videos
01:49Golgi Apparatus
As they leave the Endoplasmic Reticulum (ER), properly folded and assembled proteins are selectively packaged into vesicles. These vesicles are transported by microtubule-based motor proteins and fuse together to form vesicular tubular clusters, subsequently arriving at the Golgi apparatus, a eukaryotic endomembrane organelle that often has a distinctive ribbon-like appearance.
01:09Golgi Apparatus
Properly folded and assembled proteins are selectively packaged into vesicles that exit the ER. Motor proteins transport these vesicles to the Golgi apparatus for adding modifications that make these proteins functional at their destination.
The Golgi apparatus is a eukaryotic organelle that has a distinctive ribbon-like appearance. It is a primary sorting and dispatch station for cargo arriving from the ER. Newly arriving vesicles enter the cis face of the Golgi, closest to the ER, and are...
The Golgi apparatus is a eukaryotic organelle that has a distinctive ribbon-like appearance. It is a primary sorting and dispatch station for cargo arriving from the ER. Newly arriving vesicles enter the cis face of the Golgi, closest to the ER, and are...
01:09Golgi Apparatus
Properly folded and assembled proteins are selectively packaged into vesicles that exit the ER. Motor proteins transport these vesicles to the Golgi apparatus for adding modifications that make these proteins functional at their destination.
The Golgi apparatus is a eukaryotic organelle that has a distinctive ribbon-like appearance. It is a primary sorting and dispatch station for cargo arriving from the ER. Newly arriving vesicles enter the cis face of the Golgi, closest to the ER, and are...
The Golgi apparatus is a eukaryotic organelle that has a distinctive ribbon-like appearance. It is a primary sorting and dispatch station for cargo arriving from the ER. Newly arriving vesicles enter the cis face of the Golgi, closest to the ER, and are...
01:12Golgi Matrix Proteins
Golgi matrix proteins are a group of highly dynamic proteins that maintain the stacked structure of Golgi. These proteins adapt to rapid morphological changes of the Golgi during the cell cycle. During cell division, mild proteolysis removes these connections resulting in Golgi unstacking. In The daughter cells, these proteins help reassemble the unstacked Golgi.
One of the first identified Golgi matrix proteins was GM130, a rod-like protein located in the cis-Golgi. Subsequently, many Golgi...
One of the first identified Golgi matrix proteins was GM130, a rod-like protein located in the cis-Golgi. Subsequently, many Golgi...
01:26Transport Across the Golgi
While it is unclear how molecules move between adjacent Golgi cisternae, it is apparent that the molecules move from cis- cisterna, the entry face, to the trans- cisterna, the exit face. Experiments initially suggested vesicles that bud from one cisterna and fuse with the next cisterna to transport proteins between the cisternae. This vesicular transport model describes the Golgi apparatus as a relatively static structure with a unique enzyme composition in each cisterna. Molecules are...
01:45Vesicular Tubular Clusters
After budding out from the ER membrane, some COPII vesicles lose their coat and fuse with one another to form larger vesicles and interconnected tubules called vesicular tubular clusters or VTCs. These clusters constitute a compartment at the ER-Golgi interface known as ERGIC (Endoplasmic Reticulum Golgi Intermediate Compartment). The ERGIC is a mobile membrane-bound cargo transport system that sorts proteins secreted from ER and delivers them to the Golgi.
With the help of motor proteins such...
With the help of motor proteins such...