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Potential of vaccination to confound interpretation of real-time PCR results for equine influenza

I S Diallo1, A J Read, P D Kirkland

  • 1Biosecurity Sciences Laboratory, Health and Food Sciences Precinct, Biosecurity Queensland, Department of Employment, Economic Development and Innovation, PO Box 156, Archerfield, QLD 4108, Australia. ibrahim.diallo@deedi.qld.gov.au

The Veterinary Record
|August 5, 2011
PubMed
Abstract

No abstract available in PubMed .

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Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...