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Related Concept Videos

Yeast Signaling01:28

Yeast Signaling

Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...
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Related Experiment Video

Updated: May 30, 2026

Growth-based Determination and Biochemical Confirmation of Genetic Requirements for Protein Degradation in Saccharomyces cerevisiae
10:57

Growth-based Determination and Biochemical Confirmation of Genetic Requirements for Protein Degradation in Saccharomyces cerevisiae

Published on: February 16, 2015

Gene-dependent cell death in yeast.

X Teng1, W-C Cheng, B Qi

  • 1Department of Pharmacology and Molecular Sciences, Johns Hopkins School of Medicine, Baltimore, MD, USA.

Cell Death & Disease
|August 5, 2011
PubMed
Summary
This summary is machine-generated.

Researchers identified over 800 yeast genes that, when knocked out, increase cell survival after heat stress, suggesting these genes normally promote cell death in Saccharomyces cerevisiae.

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Last Updated: May 30, 2026

Growth-based Determination and Biochemical Confirmation of Genetic Requirements for Protein Degradation in Saccharomyces cerevisiae
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Published on: February 16, 2015

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Published on: July 20, 2019

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genetics

Background:

  • Caspase-dependent apoptosis is well-studied in multicellular organisms, but its existence in single-cell species like yeast remains unclear.
  • This uncertainty limits the use of yeast genetics to study cell death regulation.
  • Mammalian studies reveal diverse genetically encoded cell death pathways, suggesting potential yeast applications.

Purpose of the Study:

  • To investigate the prevalence of gene-dependent cell death in yeast.
  • To identify yeast genes that contribute to cell-autonomous death.
  • To leverage yeast genetics for cell death research.

Main Methods:

  • A rigorous strategy was employed, allowing time for gene-dependent events but not population evolution.
  • A ramped heat stimulus was applied to the Saccharomyces cerevisiae gene knockout collection.
  • Automated counting of microscopic colonies assessed viability and identified gene-specific survival phenotypes.

Main Results:

  • Over 800 yeast knockout strains showed significantly increased survival after heat insult.
  • This implies that the deleted genes normally contribute to cell death.
  • Identified survival phenotypes were reproducible and often persisted under alternative conditions.

Conclusions:

  • This study demonstrates that a significant number of yeast genes can contribute to programmed cell death.
  • It provides a foundation for further research into the mechanisms of yeast cell death.
  • The findings encourage the application of yeast genetics to study cell death regulation.