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Updated: May 30, 2026

Somatic Genome-Engineered Mouse Models Using In Vivo Microinjection and Electroporation

Somatic Genome-Engineered Mouse Models Using In Vivo Microinjection and Electroporation

Published on: May 5, 2023

Somatic genetics empowers the mouse for modeling and interrogating developmental and disease processes.

Sean F Landrette¹, Tian Xu

¹Department of Genetics, Howard Hughes Medical Institute, Yale University School of Medicine, Boyer Center for Molecular Medicine, New Haven, Connecticut, United States of America.

|August 5, 2011

Summary

New genetic tools, including the piggyBac transposon system, enable efficient in vivo validation of human disease genes in mice. These advances aid in modeling diseases and screening for therapeutic targets.

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Area of Science:

Genomics and Genetics
Mammalian Genetics
Molecular Biology

Background:

Genomic technologies accelerate the identification of candidate human disease genes.
Efficient in vivo validation of these genetic mutations is crucial.
Current genetic tools for in vivo validation require advancement.

Purpose of the Study:

To review advances in murine somatic mutagenesis for validating human disease genes.
To discuss the application of new tools, like the piggyBac transposon system, in human disease research.
To highlight the potential of these tools in deciphering human biology and disease.

Main Methods:

Review of current literature on murine somatic mutagenesis.
Discussion of somatic transgenesis, humanized rodents, and forward genetics.
Focus on the piggyBac transposon system for gene insertion and mutagenesis.

Main Results:

Murine somatic mutagenesis offers powerful tools for in vivo genetic studies.
Somatic transgenesis and humanized rodents facilitate disease modeling.
Forward genetics screens can identify novel disease-related mutations and pathways.

Conclusions:

Advances in murine somatic mutagenesis provide essential tools for human disease gene validation.
The piggyBac transposon system offers a versatile platform for genetic manipulation in vivo.
These genetic tools are pivotal for understanding human biology and developing therapeutic interventions.