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Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production
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Published on: September 20, 2016

Yeast bioinformatics and strain engineering resources.

Audrey L Atkin1

  • 1School of Biological Sciences, University of Nebraska - Lincoln, Lincoln, NE, USA. aatkin1@unl.edu

Methods in Molecular Biology (Clifton, N.J.)
|August 5, 2011
PubMed
Summary

Saccharomyces cerevisiae, or baker's yeast, is ideal for engineering due to its known genome and resources. This chapter details using the Saccharomyces Genome Database for gene analysis and experimental strategies.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Synthetic Biology

Background:

  • Saccharomyces cerevisiae (baker's yeast) is a key industrial microorganism.
  • It possesses a well-annotated genome and abundant molecular analysis resources.
  • These features make it highly suitable for design-based engineering applications.

Purpose of the Study:

  • To outline the utility of the Saccharomyces Genome Database (SGD).
  • To describe strategies for analyzing gene expression data.
  • To present available genetic resources for experimental work with S. cerevisiae.

Main Methods:

  • Utilizing the Saccharomyces Genome Database for gene analysis and homolog identification.
  • Implementing strategies for the integration and analysis of gene expression data.

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  • Leveraging established genetic resources for experimental procedures.
  • Main Results:

    • Demonstrated the practical application of SGD for S. cerevisiae research.
    • Provided a framework for effective gene expression data analysis.
    • Highlighted the accessibility and utility of genetic tools for yeast engineering.

    Conclusions:

    • Saccharomyces cerevisiae is a powerful platform for biotechnological advancements.
    • The Saccharomyces Genome Database is an invaluable resource for yeast research.
    • Comprehensive genetic resources facilitate experimental design and execution in S. cerevisiae.