Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Diversity of Archaea I01:30

Diversity of Archaea I

Archaea, a domain of single-celled microorganisms, are classified into five major phyla based on genetic and biochemical characteristics: Euryarchaeota, Crenarchaeota, Thaumarchaeota, Korarchaeota, and Nanoarchaeota. Among these, the phylum Euryarchaeota is notable for its remarkable diversity in morphology, metabolism, and ecological adaptations.Morphological and Metabolic DiversityMembers of Euryarchaeota exhibit a variety of cellular shapes, including rods and cocci. Their metabolic pathways...
Diversity of Archaea IV01:29

Diversity of Archaea IV

Hyperthermophilic archaea are a group of extremophiles thriving at temperatures above 80°C, often in hydrothermal vents and volcanic soils where conditions surpass the boiling point of water. At such temperatures, proteins, membranes, and DNA in most organisms degrade, but hyperthermophiles have evolved remarkable adaptations to maintain stability and function.Unique Cellular FeaturesHyperthermophilic membranes are composed of a monolayer of biphytanyl tetraether lipids, which resist thermal...
Diversity of Archaea III01:27

Diversity of Archaea III

Crenarchaeota, a prominent phylum of Archaea, is remarkable for its ability to thrive in extreme environments characterized by high temperatures and acidity. These microorganisms inhabit sulfuric hot springs, volcanic systems, and submarine hydrothermal vents, where temperatures often exceed 100°C. The unique adaptations of Crenarchaeota not only allow survival under such extreme conditions but also provide insights into the mechanisms of life in primordial Earth-like environments.Morphological...
Hyperthermophilic Bacteria01:21

Hyperthermophilic Bacteria

Domain Bacteria includes some unique hyperthermophilic species. They exhibit remarkable adaptations that enable survival in extreme environments.Thermotoga species are rod-shaped, gram-negative, non-sporulating hyperthermophiles that form a sheath-like envelope called a toga. They ferment sugars or starch, producing lactate, acetate, CO₂, and H₂, and can also grow via anaerobic respiration using H₂ and ferric iron. Found in hot springs and hydrothermal vents, over 20% of their genes show strong...
Viruses of Archaea01:29

Viruses of Archaea

Archaeal viruses play a crucial role in the ecosystems of extremophilic archaea, particularly those belonging to the phyla Euryarchaeota and Crenarchaeota. By shaping host evolution and facilitating gene transfer, these viruses influence microbial communities and contribute to genetic diversity in extreme environments. The archaea they infect thrive in acidic hot springs and hydrothermal vents characterized by high temperatures and low pH. Archaeal viruses exhibit remarkable structural...
Overview of Archaea01:29

Overview of Archaea

Archaea, named after the Archaean eon, represent a unique domain of life, distinct from bacteria and eukaryotes, with remarkable traits. Their cellular and molecular features, ecological adaptability, and industrial relevance highlight their importance in understanding life processes and leveraging biotechnology.Cellular and Molecular CharacteristicsA defining feature of archaea is their unique membrane composition. Archaeal membranes contain ether-linked isoprenoid lipids, which confer...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Preclinical characterization of the efficacy and safety of biologic N-001 as a novel pain analgesic for post-operative acute pain treatment.

Scientific reports·2023
Same author

MetFish: a Metabolomics Pipeline for Studying Microbial Communities in Chemically Extreme Environments.

mSystems·2021
Same author

Preparation, FPLC Purification and LC-FT-ICR-MS of Proteins.

Bio-protocol·2021
Same author

Efficacy of a novel lantibiotic, CMB001, against MRSA.

The Journal of antimicrobial chemotherapy·2021
Same author

Isolation, Characterization and Structure Elucidation of a Novel Lantibiotic From <i>Paenibacillus</i> sp.

Frontiers in microbiology·2020
Same author

Intracellular G-actin targeting of peripheral sensory neurons by the multifunctional engineered protein C2C confers relief from inflammatory pain.

Scientific reports·2020

Related Experiment Video

Updated: May 30, 2026

Adaptation at the Extremes of Life: Experimental Evolution with the Extremophile Archaeon Sulfolobus acidocaldarius
08:11

Adaptation at the Extremes of Life: Experimental Evolution with the Extremophile Archaeon Sulfolobus acidocaldarius

Published on: June 14, 2024

Engineering thermoacidophilic archaea using linear DNA recombination.

Yukari Maezato1, Karl Dana, Paul Blum

  • 1School of Biological Sciences, University of Nebraska, Lincoln, NE, USA.

Methods in Molecular Biology (Clifton, N.J.)
|August 5, 2011
PubMed
Summary

Thermoacidophilic archaea, like Sulfolobus solfataricus, are extremophiles valuable for enzymes and biomining. Recent genetic engineering advances enable precise genomic modifications in S. solfataricus.

More Related Videos

Subcloning Plus Insertion (SPI) - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors
09:02

Subcloning Plus Insertion (SPI) - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors

Published on: January 8, 2015

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity
09:16

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity

Published on: March 25, 2020

Related Experiment Videos

Last Updated: May 30, 2026

Adaptation at the Extremes of Life: Experimental Evolution with the Extremophile Archaeon Sulfolobus acidocaldarius
08:11

Adaptation at the Extremes of Life: Experimental Evolution with the Extremophile Archaeon Sulfolobus acidocaldarius

Published on: June 14, 2024

Subcloning Plus Insertion (SPI) - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors
09:02

Subcloning Plus Insertion (SPI) - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors

Published on: January 8, 2015

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity
09:16

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity

Published on: March 25, 2020

Area of Science:

  • Extremophile biology
  • Archaea genomics
  • Molecular biology

Background:

  • Thermoacidophilic archaea, primarily Sulfolobales within Crenarchaeota, are extremophiles with significant industrial and evolutionary applications.
  • Their hyperstable enzymes, biomining potential, and eukaryotic-like cellular mechanisms make them subjects of applied interest.
  • Sulfolobus solfataricus is a key model organism with established genetic manipulation techniques.

Purpose of the Study:

  • To present recent advancements in genetic engineering of Sulfolobus solfataricus.
  • To detail methods for site-specific genomic alterations in S. solfataricus.
  • To highlight the potential for creating novel cell lines with tailored functions.

Main Methods:

  • Utilizing homologous recombination for genome engineering.
  • Employing linear DNA for targeted genetic modifications.
  • Developing site-specific changes within the S. solfataricus genome.

Main Results:

  • Demonstrated successful site-specific genomic modifications in S. solfataricus.
  • Advanced techniques for engineering novel archaeal cell lines.
  • Expanded the toolkit for genetic manipulation in thermoacidophilic archaea.

Conclusions:

  • Homologous recombination and linear DNA are effective tools for engineering S. solfataricus.
  • These methods facilitate the creation of S. solfataricus strains with unique, engineered functions.
  • Further research can leverage these techniques for broader applications in biotechnology and evolutionary studies.