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Related Concept Videos

In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...

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Related Experiment Video

Updated: May 30, 2026

Introducing Point Mutations into Human Pluripotent Stem Cells Using Seamless Genome Editing
09:03

Introducing Point Mutations into Human Pluripotent Stem Cells Using Seamless Genome Editing

Published on: May 10, 2020

Gene editing in stem cells hits the target.

Ho Won Kim1, Clive Niels Svendsen

  • 1The Regenerative Medicine Institute, Cedars-Sinai Medical Center, 8700 Beverly Boulevard, Los Angeles, CA 90048, USA.

Cell Stem Cell
|August 6, 2011
PubMed
Summary

New gene modification techniques using zinc finger nuclease (ZFN) and helper-dependent adenovirus (HDAdV) offer efficient methods for pluripotent stem cells. These advances are poised to significantly impact regenerative medicine research and applications.

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Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
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Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

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Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation
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Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation

Published on: February 2, 2016

Related Experiment Videos

Last Updated: May 30, 2026

Introducing Point Mutations into Human Pluripotent Stem Cells Using Seamless Genome Editing
09:03

Introducing Point Mutations into Human Pluripotent Stem Cells Using Seamless Genome Editing

Published on: May 10, 2020

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation
09:51

Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation

Published on: February 2, 2016

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Stem Cell Research

Background:

  • Recent advancements in gene editing technologies are crucial for stem cell manipulation.
  • Pluripotent stem cells hold significant therapeutic potential in regenerative medicine.

Discussion:

  • Zinc finger nuclease (ZFN)-mediated gene modification provides a highly efficient method for targeted genetic alterations.
  • Helper-dependent adenovirus (HDAdV)-mediated gene delivery offers another promising avenue for efficient gene modification in stem cells.

Key Insights:

  • Both ZFN and HDAdV methods demonstrate high efficiency in modifying genes within pluripotent stem cells.
  • These technical breakthroughs facilitate precise genetic engineering of stem cells.

Outlook:

  • The described gene modification techniques are expected to accelerate progress in regenerative medicine.
  • Further development and application of these methods could lead to novel therapeutic strategies.