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Updated: May 30, 2026

An Efficient Method for Directed Hepatocyte-Like Cell Induction from Human Embryonic Stem Cells

An Efficient Method for Directed Hepatocyte-Like Cell Induction from Human Embryonic Stem Cells

Published on: May 6, 2021

Hepatic differentiation from human embryonic stem cells using stromal cells.

Young-Dong Yu¹, Ki-Hun Kim, Sung-Gyu Lee

¹Division of Hepatobiliary Surgery and Liver Transplantation, Department of Surgery, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.

The Journal of Surgical Research

|August 6, 2011

Summary

Researchers generated proliferative hepatocyte-like cells from human embryonic stem (hES) cells. These cells offer a promising in vitro model for studying liver development and potential cell therapies.

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Efficient Differentiation of Human Pluripotent Stem Cells into Liver Cells

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Published on: June 11, 2019

Area of Science:

Developmental Biology
Stem Cell Biology
Hepatology

Background:

Deriving hepatocytes from human embryonic stem (hES) cells is crucial for understanding liver organogenesis and developing cell-based therapies.
Establishing an unlimited supply of donor hepatocytes is essential for transplantation therapy.

Purpose of the Study:

To report the successful generation of hepatocyte-like cells from hES cells.
To evaluate the potential of these cells for studying liver development and for therapeutic applications.

Main Methods:

Hepatic endoderm cells were generated from 1-day-old embryoid bodies derived from hES cells by adding activin A for 5 days.
The generated hepatic endoderm cells were subsequently co-cultured with mitomycin-treated 3T3-J2 feeder cells.

Main Results:

Co-culture with feeder cells yielded hepatocyte-like cell colonies with significant proliferation potential, maintaining cultures for over 30 days.
Extensive expansion led to the co-expression of hepatic markers alpha-fetoprotein (AFP) and albumin, confirming their hepatocyte-like identity.

Conclusions:

Proliferative hepatocyte-like cells were successfully generated from hES cells.
These hES cell-derived hepatic cells serve as a valuable in vitro model for investigating hepatic stem/progenitor cell biology, including origin, self-renewal, and differentiation mechanisms.