Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Search for Light Pseudoscalar Bosons, Pair-Produced in Higgs Boson Decays in the Four-Electron Final State in Proton-Proton Collisions at sqrt[s]=13  TeV.

Physical review letters·2026
Same author

First Evidence for Mixing-Induced CP Violation in B_{s}^{0}→J/ψϕ(1020) Decays in pp Collisions at sqrt[s]=13  TeV.

Physical review letters·2026
Same author

Observation of Suppressed Charged-Particle Production in Ultrarelativistic Oxygen-Oxygen Collisions.

Physical review letters·2026
Same author

Measurement of D^{0} Meson Photoproduction in Ultraperipheral Heavy Ion Collisions.

Physical review letters·2026
Same author

Observation of tWZ Production at the CMS Experiment.

Physical review letters·2026
Same author

First Exclusive Reconstruction of the B^{*+}, B^{*0}, and B_{s}^{*0} Mesons and Precise Measurement of Their Masses.

Physical review letters·2026

Related Experiment Video

Updated: May 30, 2026

Optical Detection of E. coli Bacteria by Mesoporous Silicon Biosensors
07:22

Optical Detection of E. coli Bacteria by Mesoporous Silicon Biosensors

Published on: November 20, 2013

P450-based porous silicon biosensor for arachidonic acid detection.

A M Giovannozzi1, V E V Ferrero, F Pennecchi

  • 1Thermodynamic Division, Istituto Nazionale di Ricerca Metrologica, Strada delle Cacce 91, 10135 Torino, Italy. a.giovannozzi@inrim.it

Biosensors & Bioelectronics
|August 6, 2011
PubMed
Summary

A novel porous silicon biosensor detects arachidonic acid using a P450 enzyme. This dual-mode sensor measures both refractive index and fluorescence for enhanced accuracy in disease-related biomarker detection.

More Related Videos

Attaching Biological Probes to Silica Optical Biosensors Using Silane Coupling Agents
09:35

Attaching Biological Probes to Silica Optical Biosensors Using Silane Coupling Agents

Published on: May 1, 2012

Fabrication of polydimethylsiloxane (PDMS)-Based Flexible Surface-Enhanced Raman Scattering (SERS) Substrate for Ultrasensitive Detection
03:33

Fabrication of polydimethylsiloxane (PDMS)-Based Flexible Surface-Enhanced Raman Scattering (SERS) Substrate for Ultrasensitive Detection

Published on: November 17, 2023

Related Experiment Videos

Last Updated: May 30, 2026

Optical Detection of E. coli Bacteria by Mesoporous Silicon Biosensors
07:22

Optical Detection of E. coli Bacteria by Mesoporous Silicon Biosensors

Published on: November 20, 2013

Attaching Biological Probes to Silica Optical Biosensors Using Silane Coupling Agents
09:35

Attaching Biological Probes to Silica Optical Biosensors Using Silane Coupling Agents

Published on: May 1, 2012

Fabrication of polydimethylsiloxane (PDMS)-Based Flexible Surface-Enhanced Raman Scattering (SERS) Substrate for Ultrasensitive Detection
03:33

Fabrication of polydimethylsiloxane (PDMS)-Based Flexible Surface-Enhanced Raman Scattering (SERS) Substrate for Ultrasensitive Detection

Published on: November 17, 2023

Area of Science:

  • Biomedical Engineering
  • Biosensor Technology
  • Enzyme-based Detection

Background:

  • Arachidonic acid is implicated in diseases like Alzheimer's, liver cancer, and inflammation.
  • Accurate detection of arachidonic acid is crucial for disease diagnosis and monitoring.
  • Existing detection methods may lack specificity or sensitivity in complex biological matrices.

Purpose of the Study:

  • To develop a novel porous silicon biosensor for arachidonic acid detection.
  • To implement a new transduction method combining refractive index and fluorescence measurements.
  • To evaluate the sensor's performance, specificity, and selectivity in biological samples.

Main Methods:

  • Immobilization of cytochrome P450 BM3 haem domain (BMP) on a porous silicon surface.
  • Development of a dual-mode transduction system measuring refractive index and fluorescence intensity changes.
  • Utilizing a fluorophore-labeled BMP for enhanced fluorescence upon arachidonic acid binding.
  • Testing the biosensor's detection limit, dynamic range, and performance in plasma samples.

Main Results:

  • Achieved a detection limit of 10 μM for arachidonic acid with fluorescence detection (dynamic range 10-200 μM).
  • Refractive index measurements showed a higher detection limit of 30 μM.
  • Demonstrated high specificity and selectivity in preliminary plasma tests, even with interferents.
  • The dual-detection system showed potential for increased accuracy and broader dynamic range.

Conclusions:

  • The developed porous silicon biosensor offers a sensitive and selective method for arachidonic acid detection.
  • Simultaneous refractive index and fluorescence measurements enhance sensor accuracy and dynamic range.
  • This biosensor shows promise for clinical applications in diagnosing and monitoring diseases associated with arachidonic acid levels.