Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Automated Microbial Diagnostics

Automated Microbial Diagnostics

Automated diagnostic analyzers have transformed clinical microbiology by providing rapid and reliable methods for pathogen identification and antibiotic susceptibility testing. Among these systems, the Vitek 2 is widely used because it automates the traditionally labor-intensive processes of microbial identification (ID) and antibiotic susceptibility testing (AST), delivering standardized and timely results that are essential for effective patient care.Microbial Identification with ID CardsThe...

Rapid Identification of Pathogens

Rapid Identification of Pathogens

MALDI-TOF MS has transformed clinical microbiology by offering a rapid and reliable method for pathogen identification. The traditional approach to microbial identification typically involves time-consuming culture techniques and biochemical tests, which can delay the initiation of appropriate antimicrobial therapy. MALDI-TOF MS avoids these delays by using characteristic ribosomal protein mass patterns of microbial cells, enabling accurate species-level identification within minutes.Principle...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Maternal Cardiac Arrest During Cesarean Section in the Setting of Severe Preeclampsia and Uncontrolled Type 1 Diabetes: A Case Report.

Cureus·2026

Same author

Coronary heart disease and stroke mortality trends in Australia and New Zealand: comparison of official national mortality data and Global Burden of Disease estimates.

International journal of epidemiology·2025

Same author

Quantifying years of life lost in Australia: a multiple cause of death analysis.

International journal of epidemiology·2025

Same author

Molecular characterization of vaginal microbiota using a new 22-species qRT-PCR test to achieve a relative-abundance and species-based diagnosis of bacterial vaginosis.

Frontiers in cellular and infection microbiology·2024

Same author

Analysis of Multiple Causes of Death: A Review of Methods and Practices.

Epidemiology (Cambridge, Mass.)·2023

Same author

Quantifying cause-related mortality in Australia, incorporating multiple causes: observed patterns, trends and practical considerations.

International journal of epidemiology·2022

Search research articles

Related Experiment Video

Updated: May 30, 2026

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System

Published on: April 25, 2025

Rapid diagnostic test for identifying group B streptococcus.

Jonathan Faro¹, Allan Katz, Karen Bishop

¹Department of Obstetrics, Gynecology and Reproductive Sciences, University of Texas Health Science Center, Houston, Texas, USA. Jonathan.P.Faro@uth.tmc.edu

American Journal of Perinatology

|August 6, 2011

Summary

A new rapid diagnostic test accurately detects Group B Streptococcus (GBS) in just 4 hours. This breakthrough offers faster treatment for neonatal infections, significantly reducing diagnostic time compared to current methods.

More Related Videos

Rapid Identification of Gram Negative Bacteria from Blood Culture Broth Using MALDI-TOF Mass Spectrometry

Rapid Identification of Gram Negative Bacteria from Blood Culture Broth Using MALDI-TOF Mass Spectrometry

Published on: May 28, 2014

Related Experiment Videos

Last Updated: May 30, 2026

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System

Published on: April 25, 2025

Rapid Identification of Gram Negative Bacteria from Blood Culture Broth Using MALDI-TOF Mass Spectrometry

Rapid Identification of Gram Negative Bacteria from Blood Culture Broth Using MALDI-TOF Mass Spectrometry

Published on: May 28, 2014

Area of Science:

Microbiology
Clinical Diagnostics
Neonatal Health

Background:

Neonatal infections caused by Streptococcus agalactiae (Group B Streptococcus, GBS) lead to considerable illness and death.
Current diagnostic methods for GBS require 48-72 hours, delaying crucial antibiotic prophylaxis for early-onset neonatal GBS infection.
A rapid diagnostic test is essential for timely intervention and reducing unnecessary antibiotic use.

Purpose of the Study:

To develop and evaluate a rapid diagnostic test for the detection of Group B Streptococcus (GBS) in neonatal settings.
To assess the sensitivity and time-to-result of the novel GBS detection method.
To provide a faster alternative to conventional GBS diagnostic techniques.

Main Methods:

A stock culture of GBS was serially diluted (10^7 to 10^1 bacteria/mL).
Bacterial concentrations were inoculated onto nitrocellulose membranes (NCMs) pre-coated with rabbit anti-GBS polyclonal antibody.
Following incubation, NCMs were treated with horseradish-peroxidase-conjugated anti-GBS antibody and detected using diaminobenzidine.

Main Results:

GBS was detected at concentrations ranging from 10^7 to 10^4 bacteria/mL after 6 hours of incubation.
Detection was achieved at concentrations from 10^7 to 10^5 bacteria/mL after 4 hours.
No GBS detection occurred at the 2-hour incubation mark.
The test demonstrated reliable results as early as 4 hours, a significant improvement over the 48-72 hours needed for traditional methods.

Conclusions:

Rapid growth and detection of GBS are feasible within a 4-hour timeframe.
The developed diagnostic test provides reliable and significantly accelerated results for GBS identification.
This rapid test has the potential to optimize antibiotic prophylaxis strategies for early-onset neonatal GBS infections.