Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Enlargement of the Plasma Membrane01:22

Enlargement of the Plasma Membrane

Cell division and enlargement are processes that require precise control. The control ensures that cell division cannot proceed unless the cell has grown to a specific size. A spherical, dividing cell requires an approximately 1.6X increase in its surface area to double its volume. The secretory pathway also has a significant role in cell membrane enlargement. Secretory vesicles that bud off from the Golgi apparatus and later fuse with the plasma membrane during exocytosis are a major source of...
Introduction to Membrane Proteins01:16

Introduction to Membrane Proteins

The cell membrane, or plasma membrane, is an ever-changing landscape. It is described as a fluid mosaic where various macromolecules are embedded in the phospholipid bilayer. Among the macromolecules are proteins. The protein content varies across cell types. For example, mitochondrial inner membranes contain ~76% protein content, while myelin contains ~18% protein content. Individual cells contain many types of membrane proteins—red blood cells contain over 50—and different cell types have...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Parallel Accumulation-Mobility Aligned Fragmentation Strategy Utilizing High-Resolution Ion Mobility for High Performance Proteomics Analysis.

Molecular & cellular proteomics : MCP·2026
Same author

What Are the Practical Applications of Single-Cell Proteomics?

Proteomes·2026
Same author

Propionic Acid Outperforms Formic and Acetic Acid in MS Sensitivity for High-Flow Reversed-Phase LC-MS Bottom-Up Proteomics.

Analytical chemistry·2026
Same author

Dynamic switching of apoptosis-modulating BIM heterodimers in response to BH3 mimetics in xenograft models of hematologic malignancies.

Molecular cancer therapeutics·2026
Same author

Endosialin (CD248) Cancer Role and Therapeutics: 33 Years on.

Molecular cancer therapeutics·2026
Same author

Simultaneous single-cell proteomics and epigenetic analysis of histone deacetylase inhibition in human cells.

Communications biology·2025
Same journal

Unveiling the post translational modification code in Alzheimer's pathogenesis: crosstalk between phosphorylation and acetylation.

Expert review of proteomics·2026
Same journal

Plasma proteome profiles of standard-risk and high-risk childhood acute lymphoblastic leukemia.

Expert review of proteomics·2026
Same journal

Potential proteomic biomarkers for monitoring clinical studies in Duchenne/Becker muscular dystrophy.

Expert review of proteomics·2026
Same journal

Proteomics-based approaches to neutrophil biology.

Expert review of proteomics·2026
Same journal

Deciphering mitochondrial metabolic vulnerabilities in ovarian clear cell carcinoma with mass spectrometry-based clinical proteomics.

Expert review of proteomics·2026
Same journal

The proteomic origin of the genetic code.

Expert review of proteomics·2026
See all related articles

Related Experiment Video

Updated: May 30, 2026

Determination of Plasma Membrane Partitioning for Peripherally-associated Proteins
11:11

Determination of Plasma Membrane Partitioning for Peripherally-associated Proteins

Published on: June 15, 2018

Challenges in plasma membrane phosphoproteomics.

Benjamin C Orsburn1, Luke H Stockwin, Dianne L Newton

  • 1Drug Mechanism Group, Developmental Therapeutics Program, SAIC-Frederick Inc., NCI-Frederick, MD 21702, USA.

Expert Review of Proteomics
|August 9, 2011
PubMed
Summary
This summary is machine-generated.

Generating a comprehensive membrane phosphoproteome is challenging but crucial for understanding cell signaling. Recent advances in enrichment and fragmentation techniques improve the identification of these vital phosphopeptides.

Related Experiment Videos

Last Updated: May 30, 2026

Determination of Plasma Membrane Partitioning for Peripherally-associated Proteins
11:11

Determination of Plasma Membrane Partitioning for Peripherally-associated Proteins

Published on: June 15, 2018

Area of Science:

  • Proteomics
  • Cellular Biology
  • Biochemistry

Background:

  • Extracellular stimuli modulate plasma membrane-associated protein phosphorylation.
  • Understanding these changes is key to signal transduction and drug mechanism studies.
  • Analyzing the membrane phosphoproteome is difficult due to low protein abundance and insolubility.

Purpose of the Study:

  • To highlight recent advances in membrane phosphoproteomics.
  • To discuss improved techniques for identifying membrane phosphopeptides.
  • To assess the value of alternative fragmentation methods.

Main Methods:

  • Review of recent advances in membrane and phosphoprotein enrichment techniques.
  • Description of alternative phosphopeptide fragmentation methods.
  • Assessment of current and future applications in membrane phosphoproteomics.

Main Results:

  • Improved identification of low-abundance, insoluble membrane phosphopeptides.
  • Enhanced capabilities in analyzing the membrane phosphoproteome.
  • Evaluation of novel fragmentation techniques for phosphopeptide analysis.

Conclusions:

  • Recent technical advancements facilitate more comprehensive membrane phosphoproteomics.
  • Alternative fragmentation techniques offer promising solutions for phosphopeptide analysis.
  • These improvements are vital for advancing signal transduction and drug discovery research.