Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Human glomerular epithelial cell proteoglycans.

G J Thomas1, L Jenner, R M Mason

  • 1Department of Medicine, University of Wales College of Medicine, Royal Infirmary, Cardiff, United Kingdom.

Archives of Biochemistry and Biophysics
|April 1, 1990
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Intratumoral follicular regulatory T cells curtail anti-PD-1 treatment efficacy.

Nature immunology·2021
Same author

First Report of Wheat mosaic virus Infecting Wheat in Western Australia.

Plant disease·2019
Same author

Translational aspects in targeting the stromal tumour microenvironment: from bench to bedside.

New horizons in translational medicine·2016
Same author

Fibroblast activation and senescence in oral cancer.

Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·2016
Same author

Systematic review and meta-analysis of immunohistochemical prognostic biomarkers in resected oesophageal adenocarcinoma.

British journal of cancer·2015
Same author

Systematic review and meta-analysis of immunohistochemical prognostic biomarkers in resected oesophageal adenocarcinoma.

British journal of cancer·2015
Same journal

Cortical Cholesterol Deficit and Reduced Network Excitability in ApoE-Deficient Mice.

Archives of biochemistry and biophysics·2026
Same journal

Unraveling zinc's impact: Variable effects across concentrations in Phaseolus coccineus thylakoids.

Archives of biochemistry and biophysics·2026
Same journal

Characterizing tumor-associated endothelial cells in clear cell renal cell carcinoma via scRNA-seq and Bulk RNA-seq: construction of a prognostic risk signature.

Archives of biochemistry and biophysics·2026
Same journal

FOSB-Histone deacetylase 6 axis drives hepcidin-mediated ferroptosis in pulmonary ischemia-reperfusion injury.

Archives of biochemistry and biophysics·2026
Same journal

Sevoflurane preconditioning alleviates hypoxia-reoxygenation-induced myocardial injury via regulating the MIR210HG/miR-377-3p/PUM2 axis.

Archives of biochemistry and biophysics·2026
Same journal

Structural and kinetic analysis of human NQO1: Evidence for therapeutic inhibition by racemic warfarin.

Archives of biochemistry and biophysics·2026
See all related articles

Human glomerular epithelial cells synthesize various heparan and chondroitin sulfate proteoglycans. These molecules are released into the medium or metabolized intracellularly, indicating complex cellular processing and secretion pathways.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Extracellular Matrix Research

Background:

  • Proteoglycans are crucial components of the extracellular matrix and cell surface.
  • Human glomerular epithelial cells play a role in kidney function and filtration.
  • Understanding proteoglycan synthesis and turnover is key to comprehending cellular processes.

Purpose of the Study:

  • To isolate and characterize proteoglycans synthesized by human glomerular epithelial cells.
  • To investigate the localization, properties, and metabolic fate of these proteoglycans.

Main Methods:

  • Cell culture of human glomerular epithelial cells.
  • Isolation and characterization of synthesized proteoglycans using techniques like gel filtration (Sepharose CL-6B) and hydrophobic interaction chromatography (octyl-Sepharose).

Related Experiment Videos

  • Pulse-chase experiments to study proteoglycan metabolism and turnover.

    • Main Results:

    • Identified three distinct heparan sulfate proteoglycans (HSPG-I, HSPG-II) and heparan sulfate glycosaminoglycans (HS-GAG).
    • Characterized three chondroitin sulfate proteoglycans (CSPG-I, CSPG-II) and chondroitin sulfate glycosaminoglycans (CS-GAG).
    • Demonstrated differential localization (cell layer vs. medium) and properties (hydrophobicity) of these molecules.
    • Pulse-chase experiments revealed that proteoglycans are released into the medium or internalized and metabolized.

    Conclusions:

    • Human glomerular epithelial cells synthesize a diverse array of heparan and chondroitin sulfate proteoglycans.
    • These proteoglycans exhibit distinct biochemical properties and subcellular localization.
    • The cells actively process and secrete proteoglycans, with evidence of both direct release and intracellular metabolism.