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Related Experiment Video

Updated: May 30, 2026

Detection of Protein Palmitoylation in Cultured Hippocampal Neurons by Immunoprecipitation and Acyl-Biotin Exchange (ABE)

Detection of Protein Palmitoylation in Cultured Hippocampal Neurons by Immunoprecipitation and Acyl-Biotin Exchange (ABE)

Published on: February 18, 2013

Protein palmitoylation and subcellular trafficking.

Clara Aicart-Ramos¹, Ruth Ana Valero, Ignacio Rodriguez-Crespo

¹Departamento de Bioquímica y Biología Molecular 1, Universidad Complutense, Madrid, Spain.

Biochimica Et Biophysica Acta

|August 9, 2011

Summary

This summary is machine-generated.

Protein S-palmitoylation, a reversible protein modification, regulates protein trafficking and localization. DHHC enzymes control this process, impacting protein function and cellular transport.

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Detection of Protein S-Acylation using Acyl-Resin Assisted Capture

Detection of Protein S-Acylation using Acyl-Resin Assisted Capture

Published on: April 10, 2020

Area of Science:

Biochemistry
Cell Biology
Molecular Biology

Background:

Protein S-palmitoylation is the most common protein acylation in eukaryotes.
It's a reversible post-translational modification crucial for protein function.
Other modifications like N-myristoylation and prenylation are often irreversible.

Purpose of the Study:

To provide an overview of how protein palmitoylation regulates protein trafficking.
To highlight the role of S-palmitoylation in controlling subcellular localization.
To discuss the involvement of DHHC enzymes in this process.

Main Methods:

Review of cotransfection experiments involving DHHC protein palmitoyl transferases.
Analysis of RNA interference results.
Examination of protein trafficking and subcellular localization studies.

Main Results:

S-palmitoylation is a tightly regulated, reversible modification.
DHHC enzymes, often Golgi-localized, control subcellular trafficking of acylated proteins.
Protein palmitoylation influences protein stability, localization, and interactions.

Conclusions:

Protein S-palmitoylation is a key regulator of protein trafficking and subcellular localization.
The reversibility of S-palmitoylation allows dynamic protein shuttling between compartments.
DHHC enzymes play a critical role in mediating these palmitoylation-dependent processes.