Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Polymerase chain reaction (PCR) amplification with a single specific primer.

M Kalman1, E T Kalman, M Cashel

  • 1Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892.

Biochemical and Biophysical Research Communications
|March 16, 1990
PubMed
Summary

This study introduces a novel method for amplifying DNA fragments adjacent to a known DNA sequence. This technique enables the creation of functional primers for polymerase chain reactions.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cytological versus histological results in ASCUS cervical dysplasia - a retrospective study.

Ceska gynekologie·2026
Same author

Recent advances in biohydrogen research.

Pflugers Archiv : European journal of physiology·2017
Same author

Absence of post-lesion reactive gliosis in elasmobranchs and turtles and its bearing on the evolution of astroglia.

Journal of experimental zoology. Part B, Molecular and developmental evolution·2013
Same author

Antitussive activity of Althaea officinalis L. polysaccharide rhamnogalacturonan and its changes in guinea pigs with ovalbumine-induced airways inflammation.

Bratislavske lekarske listy·2012
Same author

Alterations of the perivascular dystrophin-dystroglycan complex following brain lesions: an immunohistochemical study in rats.

Histology and histopathology·2011
Same author

Optically adjustable light filaments generated by a compact laser convertor.

Optics express·2009

Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • Standard DNA amplification methods face challenges with unknown flanking regions.
  • Targeted DNA amplification is crucial for various molecular biology applications.

Purpose of the Study:

  • To develop a method for amplifying DNA fragments flanking a single known sequence.
  • To enable the synthesis of functional primers for polymerase chain reactions (PCR).

Main Methods:

  • The described method utilizes a known DNA sequence of sufficient length.
  • This sequence serves as a template for designing functional primers.
  • Polymerase chain reactions are employed for DNA amplification.

Main Results:

Related Experiment Videos

  • Successful amplification of DNA fragments adjacent to the known sequence was achieved.
  • The method facilitates the generation of primers suitable for PCR.

    • Conclusions:

    • The developed method offers an effective approach for amplifying flanking DNA sequences.
    • This technique has potential applications in genetic analysis and molecular cloning.