The fabrication of a piezoelectric immunosensor based on DNA-antibody conjugate layer

In this article, we report a method of antibody immobilization carried out by hybridizing DNA-antibody conjugates on a mixed self-assembled monolayer composed of DNA thiols and mercaptopropionic acid via sequence-specific hybridization. The proposed method was applied to fabricate an immunosensor for detecting human immunoglobulin G (IgG). Under the optimized experimental conditions, a wide linear range from 50.0 to 500 μg/ml was reached with a detection limit of 30.13 μg/ml. The developed immunosensor possesses advantages such as simple fabrication, wide linear range, easy regeneration, and excellent reproducibility.