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Published on: August 14, 2019
Radical scavenging activity of flavonoids from Trollius chinensis Bunge
Haiping Li1, Mingming Zhang, Guiji Ma
1Tianjin Key Laboratory of Food Biotechnology, Faculty of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin, 300134, PR China. hhppli@163.com
Objective:
Flos trollii is considered as functional tea, as well as a traditional medicinal herb, in China. In this study, total phenolic and flavonoid contents of Flos trollii were determined by a colorimetric method. The antioxidative potential of the hydroalcoholic extract of Flos trollii (FTE, extracted by alcohol:water, 80:20) was also evaluated by various antioxidant assays.
Methods:
Chemiluminescence technique was used to determine the radical scavenging activities of FTE toward different reactive oxygen species, including superoxide anion (O(-2)·), hydroxyl radical (OH·), lipid-derived radicals (R·), and singlet oxygen ((1)O(2)).
Results:
FTE could effectively scavenge O(-2)·, OH·, R·, and (1)O(2) at an efficient concentration (EC(50)) of 46, 5.64, 5.19, and 3.97 mg/mL, respectively. Moreover, the radical scavenging activities of FTE were higher than those of ascorbic acid. Further, FTE had higher 1,1 diphenyl-2-picrylhydrazyl radical (DPPH) radical-scavenging activity with EC(50) 44 mg/mL, compared with butylated hydroxytoluenesynthetic antioxidant with EC(50) 52 mg/mL.
Conclusion:
All results indicated that FTE is a powerful antioxidant, deserving of better utilization of the extracted Flos trollii as antioxidants.