Evaluating sludge minimization caused by predation and viral infection based on the extended activated sludge model No. 2d
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Updated: May 30, 2026

Estimating Virus Production Rates in Aquatic Systems
Published on: September 22, 2010
Xiaodi Hao1, Qilin Wang, Yali Cao
1Key Laboratory of Urban Stormwater System and Water Environment/R & D Centre for Sustainable Environmental Biotechnology, Beijing University of Civil Engineering and Architecture, Ministry of Education, 1 Zhanlanguan Road, Xicheng District, Beijing 100044, PR China. xdhao@hotmail.com
Abstract:
The Activated Sludge Model No. 2d (ASM2d) was extended to incorporate the processes of both predation and viral infection. The extended model was used to evaluate the contributions of predation and viral infection to sludge minimization in a sequencing batch reactor (SBR) system enriching polyphosphate-accumulating organisms (PAOs). Three individual decay processes formulated according to the general model rules were used in the extended model. The model was firstly calibrated and validated by different experimental results. It was used to evaluate the potential extent of predation and viral infection on sludge minimization. Simulations indicate that predation contributes roughly two times more to sludge minimization than viral infection in the SBR system enriching PAOs. The sensitivity analyses of the selected key parameters reveal that there are thresholds on both predation and viral infection rates, if they are too large a minimal sludge retention time is obtained and the effluent quality is deteriorating. Due to the thresholds, the contributions of predation and viral infection to sludge minimization are limited to a maximal extent of about 21% and 9%, respectively. However, it should be noted that the parameters concerning predation and viral infection were not calibrated separately by independent experiment in our study due to the lack of an effective method, especially for the parameters regarding viral infection. Therefore, it is essential to better evaluate these parameters in the future.
Insights
Predation and viral infection contribute to sludge minimization in wastewater treatment. Predation offers greater sludge reduction than viral infection, but both have limits to prevent system deterioration.
Area of Science:
- Environmental microbiology
- Wastewater treatment technologies
- Bioprocess modeling
Background:
- The Activated Sludge Model No. 2d (ASM2d) is a standard for simulating wastewater treatment processes.
- Understanding factors influencing sludge minimization is crucial for efficient wastewater treatment.
- Polyphosphate-accumulating organisms (PAOs) play a key role in biological nutrient removal.
Purpose of the Study:
- To extend the ASM2d model to include predation and viral infection processes.
- To quantify the contributions of predation and viral infection to sludge minimization in a sequencing batch reactor (SBR) system.
- To analyze the impact of these processes on system stability and effluent quality.
Main Methods:
- Extension of the ASM2d model to incorporate predation and viral infection.
- Calibration and validation of the extended model using experimental data.
- Simulation of SBR systems to evaluate sludge minimization potentials.
Main Results:
- Predation contributes approximately twice as much to sludge minimization as viral infection in PAO-enriched SBRs.
- Sensitivity analyses revealed thresholds for predation and viral infection rates, beyond which effluent quality and sludge retention time degrade.
- The maximal contribution of predation and viral infection to sludge minimization was estimated at 21% and 9%, respectively.
Conclusions:
- Predation and viral infection can significantly contribute to sludge minimization in SBRs, but their impact is limited by critical rate thresholds.
- Further research is needed to accurately determine parameters for predation and especially viral infection, potentially through independent experimental methods.
- The findings highlight the importance of considering these biological processes in optimizing wastewater treatment designs for sludge reduction.
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