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Atomic Force Microscopy

Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...

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Related Experiment Video

Updated: May 30, 2026

Active Probe Atomic Force Microscopy with Quattro-Parallel Cantilever Arrays for High-Throughput Large-Scale Sample Inspection

Active Probe Atomic Force Microscopy with Quattro-Parallel Cantilever Arrays for High-Throughput Large-Scale Sample Inspection

Published on: June 13, 2023

Development of a novel aptamer-based sensing system using atomic force microscopy.

Yusuke Miyachi¹, Chiaki Ogino, Tomokazu Amino

¹Department of Chemical Science and Engineering, Faculty of Engineering, Graduate School of Engineering, Kobe University, Rokkoudai-chou 1-1, Nada, Kobe 657-8501, Japan.

Journal of Bioscience and Bioengineering

|August 9, 2011

Summary

This study introduces a novel atomic force microscopy (AFM) sensing system using DNA aptamers to detect thrombin. The system achieved a low detection limit, showing promise for measuring various chemical compounds.

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Visualization of Recombinant DNA and Protein Complexes Using Atomic Force Microscopy

Visualization of Recombinant DNA and Protein Complexes Using Atomic Force Microscopy

Published on: July 18, 2011

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Visualization of Recombinant DNA and Protein Complexes Using Atomic Force Microscopy

Visualization of Recombinant DNA and Protein Complexes Using Atomic Force Microscopy

Published on: July 18, 2011

Area of Science:

Biotechnology
Nanotechnology
Analytical Chemistry

Background:

Atomic Force Microscopy (AFM) offers dynamic detection of surface forces.
DNA aptamers are highly specific single-stranded DNA molecules with strong target affinity.
AFM's force-sensing capability can be enhanced by aptamer specificity for biosensing.

Purpose of the Study:

To develop a novel DNA aptamer-based sensing system utilizing AFM.
To demonstrate the system's efficacy in detecting a target molecule, thrombin.
To establish a competitive assay for quantitative analysis.

Main Methods:

A competitive sensing strategy was designed using DNA aptamers immobilized on a gold chip.
Atomic force microscopy was employed to measure the adhesion force between the gold chip and the cantilever.
Varying concentrations of thrombin were introduced to observe changes in the measured force.

Main Results:

The affinity force detected by AFM decreased proportionally with increasing thrombin concentration.
A low limit of detection (LOD) of 0.2 nM for thrombin was successfully achieved.
The developed system demonstrated high sensitivity and specificity for thrombin detection.

Conclusions:

The novel DNA aptamer-AFM sensing system provides a sensitive and quantitative method for detecting target molecules.
This approach is suitable for measuring various chemical compounds, including proteins like thrombin.
The competitive assay design offers a robust platform for biosensing applications.