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Cloning, expression, and characterization of a new beta-agarase from Vibrio sp. strain CN41
Li Liao1, Xue-Wei Xu, Xia-Wei Jiang
1College of Life Sciences, Zhejiang University, Hangzhou 310058, China.
Applied and Environmental Microbiology
|August 9, 2011
Abstract:
A new agarase, AgaA(CN41), cloned from Vibrio sp. strain CN41, consists of 990 amino acids, with only 49% amino acid sequence identity with known β-agarases. AgaA(CN41) belongs to the GH50 (glycoside hydrolase 50) family but yields neoagarotetraose as the end product. AgaA(CN41) was expressed and characterized.
