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Related Experiment Video

Updated: May 30, 2026

A Microfluidic Device with Groove Patterns for Studying Cellular Behavior
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A Microfluidic Device with Groove Patterns for Studying Cellular Behavior

Published on: August 30, 2007

From screen to structure with a harvestable microfluidic device.

Vivian Stojanoff1, Jean Jakoncic, Deena A Oren

  • 1National Synchrotron Light Source, Brookhaven National Laboratories, Upton, NY 11973, USA.

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
|August 9, 2011
PubMed
Summary
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Microfluidic Crystal Former devices improve protein crystallization screening efficiency compared to traditional vapour-diffusion methods. This approach enhances crystal identification rates and facilitates direct structure determination, optimizing protein crystallization pipelines.

Area of Science:

  • Structural biology
  • Biophysics
  • Protein crystallization

Background:

  • High-throughput automation has increased the use of vapour-diffusion for protein crystallization screening.
  • However, traditional methods often fail to produce high-quality crystals for structural analysis.

Purpose of the Study:

  • To compare the efficacy of microfluidic Crystal Former devices against sitting-drop vapour-diffusion plates for protein crystallization.
  • To evaluate the direct use of crystals obtained from the Crystal Former for structure determination.

Main Methods:

  • Comparative analysis of crystal identification rates using Crystal Former and vapour-diffusion.
  • Structure determination of thaumatin and lysozyme crystals obtained in situ and post-harvesting from the Crystal Former.

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A Modular Microfluidic Technology for Systematic Studies of Colloidal Semiconductor Nanocrystals
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Related Experiment Videos

Last Updated: May 30, 2026

A Microfluidic Device with Groove Patterns for Studying Cellular Behavior
13:50

A Microfluidic Device with Groove Patterns for Studying Cellular Behavior

Published on: August 30, 2007

Window on a Microworld: Simple Microfluidic Systems for Studying Microbial Transport in Porous Media
14:25

Window on a Microworld: Simple Microfluidic Systems for Studying Microbial Transport in Porous Media

Published on: May 3, 2010

A Modular Microfluidic Technology for Systematic Studies of Colloidal Semiconductor Nanocrystals
09:58

A Modular Microfluidic Technology for Systematic Studies of Colloidal Semiconductor Nanocrystals

Published on: May 10, 2018

Main Results:

  • The Crystal Former yielded a higher number of initial crystallization conditions compared to vapour diffusion.
  • Crystals of thaumatin and lysozyme were successfully used for structure determination directly from the Crystal Former.

Conclusions:

  • Microfluidic Crystal Former devices offer improved efficiency for protein crystallization screening.
  • A multi-method crystallization strategy incorporating distinct kinetic trajectories can enhance pipeline output.